High purity heparin and production method therefor
AssigneesUniversity of Miyazaki, Fuso Pharmaceutical Industries, Ltd.
InventorsMichio Muguruma, Hiroshi Murata
Life of this patent
Estimating the term…
Abstract
The present invention provides a high purity heparin useful to be a pharmaceutical product, cosmetics, research reagent, or the like, and a method for producing the same, more specifically, a heparin which does not substantially contain a nitrous acid degradation-resistant impurity and a method for producing a heparin, comprising mixing an aqueous solution of 5 to 30% by weight of the heparin with ethanol having an amount (volume) 0.2 to 1 times the amount (volume) of the aqueous heparin solution to obtain a colloidal precipitate of heparin.
Claims8
A heparin obtainable by a method comprising mixing an aqueous solution of 5 to 30% by weight of a heparin with an organic solvent having an amount (volume) 0.2 to 1 times the amount (volume) of the aqueous heparin solution to obtain a colloidal precipitate of heparin, wherein the organic solvent is selected from ethanol, methanol, and a mixed solvent thereof,
- wherein a sodium chloride or sodium acetate salt is dissolved in a concentration of 50 to 500 mM in the aqueous heparin solution.
A method for producing a heparin, which comprises mixing an aqueous solution of 5 to 30% by weight of a heparin with an organic solvent having an amount (volume) 0.2 to 1 times the amount (volume) of the aqueous heparin solution to obtain a colloidal precipitate of heparin, wherein the organic solvent is selected from ethanol, methanol, and a mixed solvent thereof,
- wherein a sodium chloride or sodium acetate salt is dissolved in a concentration of 50 to 500 mM in the aqueous heparin solution.
A heparin obtainable by a method comprising mixing an aqueous solution of 5 to 30% by weight of a heparin with an organic solvent having an amount (volume) 0.2 to 1 times the amount (volume) of the aqueous heparin solution to obtain a supernatant fluid containing heparin, wherein the organic solvent is selected from ethanol, methanol, and a mixed solvent thereof,
- wherein a sodium chloride or sodium acetate salt is dissolved in a concentration of 50 to 5 mM in the aqueous heparin solution.
A method for producing a heparin, which comprises mixing an aqueous solution of 5 to 30% by weight of a heparin with an organic solvent having an amount (volume) 0.2 to 1 times the amount (volume) of the aqueous heparin solution to obtain a supernatant fluid containing heparin, wherein the organic solvent is selected from ethanol, methanol, and a mixed solvent thereof,
- wherein a sodium chloride or sodium acetate salt is dissolved in a concentration of 50 to 500 mM in the aqueous heparin solution.
Dependencies are inferred from the claim text.