Host cell lines expressing recombinant GDF-5 protein
AssigneeWarsaw Orthopedic, Inc.
InventorsDavid S. Scher, Roger E. Harrington
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Abstract
Expression vector systems are provided for increased production of a recombinant GDF-5 (rhGDF-5) protein. Also provided are transformed host cells that were engineered to produce and express high levels of rhGDF-5 protein. Methods for production and high expression of rhGDF-5 protein are disclosed herein. The methods of enhancing production and protein expression of rhGDF-5 protein as disclosed are cost-effective, time-saving and are of manufacturing quality.
Claims7
A host cell line, the cell line comprising an inclusion body comprising an isolated rhGDF-5 protein, wherein the host cell includes an expression vector comprising a polynucleotide encoding for an rhGDF-5 protein under the control of a T5 promoter, the rhGDF-5 protein comprising the amino acid sequence of SEQ ID NO:
- 4.
A method of producing an rhGDF-5 protein, the method comprising providing a host cell line having an expression vector comprising a polynucleotide encoding for the rhGDF-5 protein under the control of a T5 promoter;
- and recovering the rhGDF-5 protein from the host cell line, wherein the rhGDF-5 protein comprises the amino acid sequence of SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.