Serum albumin binding molecules
AssigneeBristol-Myers Squibb Company
InventorsMichael L. Gosselin, David Fabrizio, Joanna F. Swain, Tracy Mitchell, Ray Camphausen, Sharon T. Cload, Eric Furfine, Paul E. Morin, Ranjan Mukerjee, Simeon I. Taylor
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Abstract
The present invention relates to an antibody-like protein based on the tenth fibronectin type III domain (10Fn3) that binds to serum albumin. The invention further relates to fusion molecules comprising a serum albumin-binding 10Fn3 joined to a heterologous protein for use in diagnostic and therapeutic applications.
Claims19
A nucleic acid encoding a polypeptide comprising a fibronectin type III tenth (10Fn3) domain, wherein the 10Fn3 domain binds to domain 1 or 2 of human serum albumin with a KD of 1 μM or less, and wherein the 10Fn3 domain comprises a modified amino acid sequence in one or more of the BC, DE and FG loops relative to the wild-type human 10Fn3 domain (SEQ ID NO:
- 1), and wherein the polypeptide comprises an amino acid sequence at least 75% identical to an amino acid sequence selected from SEQ ID NOs:
- 4, 8, 12, 16, 20, and 24-44.
A nucleic acid encoding a fusion polypeptide comprising a fibronectin type III tenth (10Fn3) domain and a heterologous protein, wherein the 10Fn3 domain binds to human serum albumin with a KD of 1 μM or less and comprises an amino acid sequence at least 75% identical to an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 4, 8, 12, 16, 20, and 24-44.
Dependencies are inferred from the claim text.