MSP nanopores and related methods
AssigneesUniversity of Washington, The UAB Research Foundation
InventorsJens H. Gundlach, Michael Niederweis, Thomas Z. Butler, Mikhail Pavlenok, Mark A. Troll, Suja Sukumaran
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Abstract
Provided herein are Mycobacterium smegmatis porin nanopores, systems that comprise these nanopores, and methods of using and making these nanopores. Such nanopores may be wild-type MspA porins, mutant MspA porins, wild-type MspA paralog porins, wild-type MspA homolog porins, mutant MspA paralog porins, mutant MspA homolog porins, or single-chain Msp porins. Also provided are bacterial strains capable of inducible Msp porin expression.
Claims22
A Mycobacterium smegmatis porin A (MspA) comprising an MspA monomer, wherein the MspA monomer comprises a variant of the sequence in SEQ ID NO:
- 1, and wherein the variant comprises (i) amino acids with a neutral charge at positions 90 and 91 and (ii) an amino acid with a positive charge at one or more of amino acid positions 118, 134, and 139.
Dependencies are inferred from the claim text.