Back to results
US 9,540,416 B2Granted
Purification process
AssigneeGlaxosmithkline Biologicals SA
InventorsJuan Jose Diaz Garcia, Margit Holzer
Life of this patent
Estimating the term…
Abstract
The present disclosure concerns methods for producing purified saponins, such as QS-21, which reduces the number of necessary lyophilization cycles to one by a step of solvent exchange and optional subsequent exposure of the purified saponin product to at least one exposure to dry gas followed by exposure to a vacuum to further rid the purified saponin product of residual solvent molecules.
Claims29
A method for purifying at least one saponin in a solution comprising the steps of:
- a) providing at least one saponin in a first solvent comprising a solubilizing component;
- b) replacing at least a portion of the solubilizing component with an exchange solvent, thereby producing a replaced solvent;
- and
- c) removing the replaced solvent, to produce a dried saponin product comprising at least one saponin, wherein the first solvent comprises at least partially purified QS-21;
- and the first solvent comprises at least 22% volume/volume acetonitrile;
- and the replaced solvent comprises no more than 21% volume/volume acetonitrile.
3 more elements
Dependencies are inferred from the claim text.