Analyte injection system
AssigneeCaliper Life Sciences, Inc.
InventorsCharles Park, Persefoni Kechagia, Michael Spaid, Morten Jensen, Irina G. Kazakova, Josh Molho
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Abstract
This invention provides methods and systems for injection of analytes into a separation channel for resolution and detection. Samples can be preconditioned and concentrated by isotachophoresis (ITP) before the injection is triggered by a detected voltage event. Separation of analytes from other sample constituents can be enhanced using skewing channel ITP.
Claims21
A method of separating an analyte of interest from one or more sample constituents not of interest, the method comprising:
- stacking the analyte of interest between a first end and a second end of a first channel by isotachophoresis, the first channel further comprising a skewing channel segment between the first end and the second end;
- skewing the analyte and the one or more sample constituents not of interest by flowing the analyte and the one or more sample constituents not of interest through the skewing channel segment toward the second end;
- stacking the skewed analyte one or both of while within and after exiting the skewing channel segment to form a focused analyte band;
- and
- producing and maintaining axial diffusion of the skewed one or more sample constituents not of interest.
A method of separating an analyte of interest from one or more sample constituents not of interest, the method comprising:
- stacking the analyte of interest and the one or more sample constituents not of interest to form a compact analyte band and a compact constituents not of interest band;
- skewing the compact analyte band to form a skewed analyte band and skewing the compact constituents not of interest band to form a skewed constituents not of interest band;
- and
- stacking the skewed analyte band and the skewed constituents not of interest band to form a focused analyte band separate from an axially dispersed constituents not of interest band.
Dependencies are inferred from the claim text.