Method to increase detection efficiency of real time PCR microarray by quartz material
AssigneeHoneywell International Inc.
InventorsZhenhong Sun, Tao Pan, Xuanbin Liu
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Abstract
A reactor for the quantitative analysis of target nucleic acids using an evanescent wave detection technique and a method for the quantitative analysis of target nucleic acids are provided. The reactor includes a substrate with a cavity, a buffer layer arranged over the substrate, a quartz cover plate arranged over the buffer layer, and inlet and outlet ports. The reactor is thermally and chemically stable for PCR processing and suitable for an evanescent wave detection technique.
Claims12
A reactor for the quantitative analysis of target nucleic acids, comprising:
- a substrate having a first planar opposing surface and a second planar opposing surface, the first planar opposing surface of the substrate having a cavity;
- a buffer layer comprising a water-impermeable sealant arranged over the first planar surface of the substrate;
- a quartz cover plate modified with amino-silane and a functional group, wherein the cover plate is arranged over the buffer layer, the cover plate in combination with the cavity and buffer layer defining a reaction chamber;
- and
- nucleic acid probes tethered to the interior surface of the quartz cover plate in a known, two-dimensional pattern;
- wherein the quartz cover plate has less fluorescent background than optical glass k9 for quantitative analysis of target nucleic acids by an evanescent wave detection technique.
A reactor for the quantitative analysis of target nucleic acids, comprising:
- a substrate having a first planar opposing surface and a second planar opposing surface, the first planar opposing surface of the substrate having a cavity;
- a buffer layer arranged over the first planar surface of the substrate;
- a quartz cover plate arranged over the buffer layer, the cover plate in combination with the cavity and buffer layer defining a reaction chamber, wherein a surface of the cover plate is modified with amino-silane and further modified with thiocyanate functional groups, and wherein the surface of the cover plate includes unreacted SCN groups that are blocked;
- nucleic acid probes tethered to the interior surface of the quartz cover plate in a known, two-dimensional pattern;
- and
- at least one inlet port and at least one outlet port communicating with the reaction chamber through the substrate enabling the passage of fluid from an external source into and through the reaction chamber, and thereby defining a fluid flow path;
- wherein the quartz cover plate has less fluorescent background than optical glass k9 for quantitative analysis of target nucleic acids by an evanescent wave detection technique.
Dependencies are inferred from the claim text.