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AssigneeNational Research Council of Canada
InventorsSubash Sad, Lakshmi Krishnan, Fanny Tzelepis, Valeria Alcon, Kevin G. Young
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The present invention relates to a recombinant bacterium expressing an antigen that is translocated to the cytosol of a host organism, and uses thereof. To this end, the present invention provides a recombinant bacterium comprising a nucleic acid encoding an antigen that is translocated to the cytosol of a host cell utilizing Type III secretion system. The recombinant bacterium is generally chosen from intracellular pathogens that reside in the phagosome and fail to induce rapid T cell activation. The translocated antigen may be a viral antigen, a bacterial antigen, or a tumor antigen. Methods of imparting immunity using the recombinant bacterium are also provided.
A method of imparting immunity against a tumor, a bacterium, or a virus in a subject, the method comprising administering to said subject a recombinant Salmonella bacterium comprising a nucleic acid encoding a fusion protein comprising an antigen of the tumour, an antigen of the bacterium, or an antigen of the virus, and an N-terminal secretion signal from a type III secretion domain, wherein the N-terminal secretion signal consists of SEQ ID NO:
Dependencies are inferred from the claim text.