Individualized bacterial treatment of pancreatic cancer
AssigneeAntiCancer, Inc.
InventorsMing Zhao, Fuminari Uehara
Life of this patent
Estimating the term…
Abstract
An individualized bacterial treatment of cancer is provided. The treatment includes a strain of bacteria modified by in-vivo passage through tumor grafts in experimental animals, where the modified strain exhibits enhanced cancer cell-targeting of a specific malignancy arising in a unique individual to the corresponding parent strain of bacteria. The treatment uses this modified strain for the treatment human solid-tumor malignancies by inoculating an individual with a quantity of the strain; and repeating inoculations at periodic intervals where repeated inoculations tend to progressively eliminate the solid tumor malignancy in the individual.
Claims21
A method for creating a strain of enhanced cancer cell-targeting bacteria for individualized cancer therapy, said method comprising:
- (a) harvesting cancer cells from an individual;
- (b) transplanting a quantity of said cancer cells into a quantity of experimental animals;
- (c) incubating the experimental animals to establish a cancer tissue graft within the experimental animals;
- (d) inoculating a Salmonella typhimuriumA1 into a said experimental animal containing established said cancer tissue graft;
- (e) removing said cancer tissue graft from said experimental animal inoculated with said Salmonella typhimuriumA1 following a period of incubation of the inoculated said experimental animal;
- (f) extracting a Salmonella typhimuriumA1-R from said cancer tissue graft removed from the experimental animal inoculated with said Salmonella typhimuriumA1-R;
- (g) incubating said Salmonella typhimuriumA1-R under conditions suitable for bacterial multiplication;
- (h) selecting a said Salmonella typhimuriumA1-R;
- (i) incubating the selected said Salmonella typhimuriumA1-R under conditions suitable for bacterial multiplication;
- (j) inoculating a quantity of selected said Salmonella typhimuriumA1-R into a second said experimental animal containing established said cancer tissue graft;
- (k) removing said cancer tissue graft from the said experimental animal inoculated with a quantity Salmonella typhimuriumA1-R following a period of incubation;
- (l) extracting Salmonella typhimuriumA1-R from said cancer tissue graft removed from the experimental animal;
- (m) incubating said bacteriaR1 under conditions suitable for bacterial multiplication;
- and
- (n) selecting a Salmonella typhimuriumR1.
Dependencies are inferred from the claim text.