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US 9,538,945 B2Granted
Desorption electrospray ionization sampling without damaging an in vivo tissue sample
AssigneePurdue Research Foundation
InventorsRobert Graham Cooks, Zheng Ouyang, Chien-Hsun Chen, Ziqing Lin, Livia Schiavinato Eberlin
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Abstract
The invention generally relates to enclosed desorption electrospray ionization probes, systems, and methods. In certain embodiments, the invention provides a source of DESI-active spray, in which a distal portion of the source is enclosed within a transfer member such that the DESI-active spray is produced within the transfer member.
Claims20
A method for analyzing in vivo tissue, the method comprising:
- generating from a sampling probe, a liquid droplet spray discharge that is non-toxic to in vivo tissue;
- directing the liquid droplet spray discharge onto the in vivo tissue, wherein the liquid droplet spray discharge from the sampling probe desorbs and ionizes an analyte from the in vivo tissue in a manner that does not damage the in vivo tissue;
- transferring the ions of the analyte to a mass spectrometer;
- and
- analyzing the ions of the analyte in the mass spectrometer, thereby analyzing the in vivo tissue.
2 more elements
A system for analyzing an in vivo tissue, the system comprising:
- a discharge source that operates without voltage and that produces a liquid droplet spray discharge that desorbs and ionizes an analyte from a sample in a manner that does not damage the in vivo tissue;
- a transfer member;
- a mass spectrometer;
- and
- a pump;
- wherein the system is configured such that the transfer member is coupled to the mass spectrometer and the pump, a distal portion of the source is enclosed within the transfer member such that the liquid droplet spray discharge is produced within the transfer member in a first direction and its velocity is reduced by the pump, which produces a gas flow in the transfer member in a direction opposite that of the first direction, and a gas flow produced by the system transfers ions through the transfer member to the mass spectrometer.
3 more elements
Dependencies are inferred from the claim text.