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US 9,534,230 B2Granted
Stress resistant plants
AssigneeBayer CropScience AG
InventorsMarc De Block, Michael Metzlaff, Véronique Gossele
Life of this patent
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Abstract
Stress tolerance in plants and plant cells is achieved by using nucleotide sequences encoding enzymes involved in the NAD salvage synthesis pathway and/or the NAD de novo synthesis pathway e.g. for overexpression in plants.
Claims12
A method for obtaining a plant with increased stress resistance comprising
- a. introducing a chimeric gene into cells of a plant to obtain transgenic cells, said chimeric gene comprising the following operably linked DNA fragments:
- i. a plant-expressible promoter;
- ii. a DNA region coding for a plant-functional enzyme of the nicotinamide adenine dinucleotide (NAD) salvage synthesis pathway, wherein said DNA region codes for, nicotinic acid mononucleotide adenyl transferase comprising the amino sequence of SEQ ID No.:
- 6 or SEQ ID No.:
- 8;
- and
- iii. a 3′end region involved in transcription termination and polyadenylation;
- b. regenerating said transgenic cells to obtain a population of transgenic plants;
- and
- c. selecting a plant from said population of transgenic plants which exhibits increased stress resistance, exhibits a reduced level of reactive oxygen species, or maintains a high level of NADH under stress conditions when compared to a wild-type plant.
7 more elements
A chimeric gene comprising the following operably linked DNA fragments:
- i. a plant-expressible promoter;
- ii. a DNA region coding for a plant-functional enzyme of the nicotinamide adenine dinucleotide (NAD) salvage synthesis pathway, wherein said DNA codes for nicotinic acid mononucleotide adenyl transferase comprising the amino sequence of SEQ ID No.:
- 6 or SEQ ID No.:
- 8;
- and
- iii. a 3′end region involved in transcription termination and polyadenylation.
3 more elements
Dependencies are inferred from the claim text.