Polypeptides having peroxygenase activity
AssigneeNovozymes A/S
InventorsSara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
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Abstract
The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from Daldinia caldariorum.
Claims24
A method of producing a polypeptide having peroxygenase activity, comprising:
- (a) cultivating a recombinant host cell which comprises a nucleic acid construct or expression vector comprising a polynucleotide encoding the polypeptide, wherein the polypeptide has at least 80% sequence identity to amino acids 1 to 244 of SEQ ID NO:
- 2 and the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in the recombinant host cell under conditions conducive for production of the polypeptide;
- and
- (b) recovering the polypeptide.
A detergent composition, comprising a surfactant and a polypeptide having peroxygenase activity and having at least 80% sequence identity to amino acids 1 to 244 of SEQ ID NO:
- 2.
A method for hydroxylation in position 2 or 3 of either end of a substituted or unsubstituted, linear or branched, aliphatic hydrocarbon having at least 3 carbons and having a hydrogen attached to the carbon in position 2 or , comprising contacting the aliphatic hydrocarbon with hydrogen peroxide and a polypeptide having peroxygenase activity and having at least 80% sequence identity to amino acids 1 to 244 of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.