Recombinant cellulosome complex and uses thereof
AssigneeCarbios
InventorsRoberto Mazzoli, Enrica Pessione, Loredana Tarraran, Chiara Gandini
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Abstract
The present invention relates to a polynucleotide encoding a recombinant scaffolding polypeptide comprising at least a signal peptide, a Cellulose Binding Domain, two cohesin domains and an S-layer Homology domain, wherein said isolated polynucleotide preferably comprises all or an active part of the nucleotide sequence as set forth in SEQ ID NO:2. The present invention further relates to vectors comprising such polynucleotides, recombinant lactic acid bacteria, and method for degrading a cellulosic biomass using such recombinant lactic acid bacteria.
Claims10
An isolated polynucleotide encoding a recombinant scaffolding polypeptide comprising a signal peptide, a Cellulose Binding Domain, one or more S-layer Homology (SLH) domains and from 2 to 8 cohesin domains,
- wherein the sequence of the signal peptide comprises SEQ ID NO:
- 21 or 45, the sequence of the Cellulose Binding Domain comprises SEQ ID NO:
- 22, the sequences of the cohesin domains independently comprise any one of SEQ ID NOs:
- 3 to 11, and the sequences of the SLH domains independently comprise any one of SEQ ID NOs:
- 12 to 15 and 35 to 37.
Dependencies are inferred from the claim text.