Methods for eliminating at least a substantial portion of a clonal antigen-specific memory T cell subpopulation
AssigneeLife Technologies Corporation
InventorsRonald Berenson, Mark Bonyhadi, Dale Kalamasz
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Abstract
The present invention relates generally to methods for stimulating T cells, and more particularly, to methods to eliminate undesired (e.g., autoreactive, alloreactive, pathogenic) subpopulations of T cells from a mixed population of T cells, thereby restoring the normal immune repertoire of said T cells. The present invention also relates to compositions of cells, including stimulated T cells having restored immune repertoire and uses thereof.
Claims6
A method for activating and expanding naïve T cells present in a mixed population of T cells comprising naïve T cells and antigen-specific memory T cells, the method comprising:
- contacting the mixed population of T cells with a surface having attached thereto agents with binding activity to cell surface moieties on the surfaces of T cells in the mixed population,
- thereby (a) expanding naïve T cells, and (b) deleting antigen-specific memory T cells present in the mixed population of T cells,
- wherein the agents having binding affinity for CD3 protein complexes and CD28 proteins, and
- wherein the ratio of surface to cells is at least 5:1.
Dependencies are inferred from the claim text.