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US 9,523,685 B2Granted
Multiplex method for detecting an infection
AssigneeBio-Rad Innovations
InventorsChristine Charpentier, Stephane Gadelle, Nadine Lambert, Amparo Sanjuan
Life of this patent
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Abstract
The invention relates to a method for the in vitro diagnostic detection of an infection with a microorganism, comprising placing a biological sample, in a single assay receptacle, in the presence of particles, each carrying at least one specific detectable physical parameter, and belonging to at least two different groups, one of the groups carrying an anti-IgM capture antibody and the other group carrying a capture antigen derived from said microorganism.
Claims16
An in vitro method for the detection of antibodies directed against a microorganism present in a biological sample comprising:
- a) forming a mixture, in a single assay receptacle, comprising a biological sample and at least two groups of particles, said at least two groups of particles comprising a first particle group carrying an anti-IgM capture antibody that specifically binds to IgM and a second particle group carrying a capture antigen derived from a microorganism,
- b) incubating the mixture under conditions which allow the formation of immunocomplexes on each group of particles,
- c) eliminating from the mixture of step b) the immunoglobulins of the biological sample which have not bound to particles,
- d) incubating the particles of step c) with at least one labelled conjugate to form an immunocomplex-conjugate mixture on said particles, said at least one labelled conjugate comprising a detection antigen derived from said microorganism conjugated to a label,
- e) eliminating from the mixture of step d) the detection antigen not bound to the immunocomplex conjugates on the particles, and
- f) simultaneously detecting immunocomplexes formed on each particle group with a detector capable of differentiating particle groups, and determining the presence or the amount of immunoglobulin G (IgG) or total immunoglobulin and immunoglobulin M (IgM) directed against said microorganism in said biological sample.
3 more elements
A method for the in vitro detection of an infection with a microorganism comprising:
- a) forming a mixture, in a single assay receptacle, comprising a biological sample and at least two groups of particles, said at least two groups of particles comprising a first particle group carrying an anti-IgM capture antibody that specifically binds to IgM and a second particle group carrying a capture antigen derived from a microorganism,
- b) incubating the mixture under conditions which allow the formation of immunocomplexes on each group of particles,
- c) eliminating from the mixture of step b) the immunoglobulins of the biological sample which have not bound to particles,
- d) incubating the particles of step c) with at least one labelled conjugate to form an immunocomplex-conjugate mixture on said particles, said at least one labelled conjugate comprising a detection antigen derived from said microorganism conjugated to a label,
- e) eliminating from the mixture of step d) the detection antigen not bound to the immunocomplex conjugates on the particles, and
- f) simultaneously detecting immunocomplexes formed on each particle group with a detector capable of differentiating particle groups, the presence or the amount of immunoglobulin (IgG) or total immunoglobulin and/or immunoglobulin M (IgM) directed against said microorganism indicating an infection by said microorganism.
3 more elements
A method for the in vitro diagnostic detection of an infection with a microorganism comprising:
- a) forming a mixture, in a single assay receptacle, comprising a biological sample and at least two groups of particles, said at least two groups of particles comprising a first particle group carrying an anti-IgM capture antibody that specifically binds to IgM and a second particle group carrying a capture antigen derived from a microorganism,
- b) incubating the mixture under conditions which allow the formation of immunocomplexes on each group of particles,
- c) eliminating from the mixture of step b) the immunoglobulins of the biological sample which have not bound to particles,
- d) incubating the particles of step c) with at least one labelled conjugate to form an immunocomplex-conjugate mixture on said particles, said at least one labelled conjugate comprising a detection antigen derived from said microorganism conjugated to a label,
- e) eliminating from the mixture of step d) the detection antigen not bound to the immunocomplex conjugates on the particles, and
- f) simultaneously detecting immunocomplexes formed on each particle group with a detector capable of differentiating particle groups, the presence or the amount of immunoglobulin G (IgG) or total immunoglobulin and/or immunoglobulin M (IgM) directed against said microorganism indicating an infection by said microorganism.
3 more elements
Dependencies are inferred from the claim text.