Phosphodeoxyribosyl transferase mutant enzymes and uses thereof
AssigneeCentre National de la Recherche Scientifique (CNRS)
InventorsPierre-Alexandre Kaminski, Gilles Labesse
Life of this patent
Estimating the term…
Abstract
The present invention relates to enzyme mutants obtained from the nucleoside deoxyribosyltransferase (NDT) of Lactobacilli bacteria. These mutants are surprisingly able to exchange deoxyribose mono-, bi- and triphosphate between various nucleobases. In vitro syntheses have been successfully performed with several nucleobase analogs. These enzyme mutants provide new tools to synthesize, in vitro and/or in vivo, dNTPs or analogs thereof.
Claims24
A mutant phosphodeoxyribosyltransferase enzyme comprising amino acid sequence SEQ ID NO:1, in which the Aspartic acid at position 92 is replaced by a Serine (S) or a Glycine (G), provided that, when the Aspartic acid at position 92 is replaced by a Glycine (G), the Valine amino acid at position 93 is replaced by a Threonine (T), or a Proline (P), or a Serine (S) or a Glycine (G).
Dependencies are inferred from the claim text.