US 9,523,083 B2 · Biochemical synthesis of 1,4-butanediamine

Abstract

Process for biochemical synthesis of 1,4-butanediannine in a microorganism having an increased level of an ornithine decarboxylase activity as compared to the native level of the ornithine decarboxylase activity, in which the increased ornithine decarboxylase activity is obtained by overexpression of an ornithine decarboxylase encoding gene with increased translational and/or transcriptional efficiency. The process is performed by excreting 1,4-butanediamine produced in the microorganism into a fermentation broth, and recovering the 1,4-butanediannine from the fermentation broth. The increased translational and/or transcriptional efficiency is obtained by the use of a strong, regulated promoter consisting of an isopropvl-B-D-thiogalactoside (IPTG) inducible strong promoter.

Claims2

Dependent claims

A method for production of 1,4-butanediamine (DAB) in a fermentation process by a genetically modified Escherichia coli (E. coli) bacterium which has the ability to produce ornithine decarboxylase (ODC), wherein said E. coli bacterium has been modified to enhance an activity of ODC, the method comprising:

(i) transforming said E. coli bacterium with a plasmid comprising an ornithine decarboxylase speF gene from Enterobacteracea family under the control of an isopropyl-β-D-thiogalactopyranoside (IPTG) inducible strong promoter,
(ii) culturing the modified E. coli bacterium of (i) in a glucose and mineral salt containing fermentation broth to allow overexpression of the ornithine decarboxylase speF, and
(iii) collecting the 1,4-butanediamine (DAB) produced and excreted by the modified E. coli bacterium into the fermentation broth, wherein the accumulation of DAB into the fermentation broth is at least 715 mg/L.

Dependencies are inferred from the claim text.

Biochemical synthesis of 1,4-butanediamine

Life of this patent

Biochemical synthesis of 1,4-butanediamine

Life of this patent

Abstract

Claims2