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US 9,522,929 B2Granted
Non-caloric sweetener
AssigneeConagen Inc.
InventorsGuohong Mao, Venkata Sai Prakash Chaturvedula, Oliver Yu
Life of this patent
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Abstract
Disclosed is a steviol glycoside referred to as rebaudioside D3. Rebaudioside D3 has five β-D-glucosyl units connected to the aglycone steviol. Also disclosed are methods for producing rebaudioside D3, a UDP-glycosyltransferase fusion enzyme, and methods for producing rebaudioside D and rebaudioside E.
Claims5
A method for synthesizing rebaudioside D3, the method comprising:
- preparing a reaction mixture comprising stevioside;
- a substrate selected from the group consisting of sucrose, uridine diphosphate and uridine diphosphate-glucose;
- and a UDP-glycosyltransferase fusion enzyme comprising a uridine diphospho glycosyltransferase domain coupled to a sucrose synthase domain;
- and incubating the reaction mixture for a sufficient time to produce rebaudioside D3, wherein the UDP-glycosyltransferase is a Oryza sativa uridine diphospho glycosyltransferase EUGT11 comprising the amino acid sequence of SEQ ID NO:1, wherein a glucose is covalently coupled to the stevioside to produce a rebaudioside E intermediate, and wherein a glucose is covalently coupled to the rebaudioside E intermediate to produce rebaudioside D3.
1 more element
Dependencies are inferred from the claim text.