# US 9,518,919 B2 · Apparatus and method for measuring hemoglobin concentration within blood using light and heat light scattering > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 9,518,919 B2 - Document ID: US9518919B2 - Status: Granted - Kind: B2 - Application number: 14/866,410 - Filed: 2015-09-25 - Published: 2016-03-31 - Granted: 2016-12-13 - Earliest claimed: 2014-09-26 - Assignees: Industry-Academic Cooperation Foundation, Yonsei University - Applicants: Industry-Academic Cooperation Foundation Yonsei University - Inventors: Chulmin Joo; Dong Hak Lee; Ui Han Kim - Web page: https://patentgrep.com/patent/US9518919B2 ## CPC - G01N 21/51: inside a container, e.g. in an ampoule (G01N21/53 takes precedence) - G01N 21/1717: {with a modulation of one or more physical properties of the sample during the optical investigation, e.g. electro-reflectance} - G01N 33/49: Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)} - G06T 7/0012: {Biomedical image inspection} - G01N 2021/1731: {Temperature modulation} - G01N 2021/4721: {using a PSD} - G06T 2207/20056: Discrete and fast Fourier transform, [DFT, FFT] - G06T 2207/30024: Cell structures in vitro; Tissue sections in vitro - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - G06T: IMAGE DATA PROCESSING OR GENERATION, IN GENERAL ## Abstract An apparatus for measuring a hemoglobin concentration includes a reference light source unit, a light and heat light source unit configured to emit a light and heat beam for generating a light and heat effect, an accommodation unit configured to accommodate obtained blood samples, an image acquisition unit configured to write a primary pattern formed after the reference beam emitted by the reference light source unit is incident on the accommodation unit and a secondary pattern formed under an influence of a light and heat effect after the light and heat beam emitted by the light and heat light source unit is incident on the accommodation unit, and a concentration calculation unit electrically connected to the image acquisition unit and configured to calculate a hemoglobin concentration based on a difference between the primary pattern and the secondary pattern. ## Claims ### Claim 1 An apparatus for measuring a hemoglobin concentration, comprising: a reference light source unit (100); a light and heat light source unit (200) configured to emit a light and heat beam for generating a light and heat effect; an accommodation unit (300) configured to accommodate obtained blood samples; an image acquisition unit (400) configured to write a primary pattern formed after the reference beam emitted by the reference light source unit (100) is incident on the accommodation unit (300) and a secondary pattern formed under an influence of a light and heat effect after the light and heat beam emitted by the light and heat light source unit (200) is incident on the accommodation unit (300); and a concentration calculation unit (500) electrically connected to the image acquisition unit and configured to calculate a hemoglobin concentration based on a difference between the primary pattern and the secondary pattern. ### Claim 2 (depends on 1) The apparatus of claim 1, wherein a wavelength of the reference beam comprises a first wavelength region which is a wavelength region in which hemoglobin is less absorbed compared to the light and heat beam. ### Claim 3 (depends on 2) The apparatus of claim 2, wherein the first wavelength region is 600 nm to 1100 nm. ### Claim 4 (depends on 1) The apparatus of claim 1, wherein a wavelength of the light and heat beam comprises a second wavelength region absorbed by hemoglobin. ### Claim 5 (depends on 4) The apparatus of claim 4, wherein the second wavelength region is 300 nm to 600 nm. ### Claim 6 (depends on 1) The apparatus of claim 1, wherein the reference light source unit (100) comprises any one of a laser, a laser diode and an LED. ### Claim 7 (depends on 1) The apparatus of claim 1, wherein the light and heat light source unit (200) comprises any one of a diode pumped solid state (DPSS) laser, an LED, and a laser diode. ### Claim 8 (depends on 1) The apparatus of claim 1, wherein the accommodation unit (300) comprises a transparent portion generally or partially so that the light and heat beam is transferred to the blood. ### Claim 9 (depends on 1) The apparatus of claim 1, wherein the image acquisition unit (400) comprises any one of a complementary metal-oxide semiconductor (CMOS), a charge coupled apparatus (CCD), a single photodiode, a photodiode array, and a position sensitive detector. ### Claim 10 (depends on 1) The apparatus of claim 1, wherein the light and heat beam emitted by the light and heat light source unit is changed into a thick and parallel pencil of rays through a beam expander before the light and heat beam is incident on the accommodation unit. ### Claim 11 (depends on 1) The apparatus of claim 1, further comprising a concave lens disposed between the accommodation unit and the image acquisition unit so that expanded primary and secondary scattering patterns are written in the image acquisition unit. ### Claim 12 (depends on 1) The apparatus of claim 1, wherein the light and heat beam comprises a beam having intensity varied over time. ### Claim 13 (depends on 12) The apparatus of claim 12, wherein the concentration calculation unit (500) forms graphs of the primary and secondary patterns, obtains a first measurement value of a peak by performing Fourier transform on a graph of a phase shift over given time, and calculates a hemoglobin concentration based on an associative relation between a hemoglobin concentration and the first measurement value. ### Claim 14 (depends on 1) The apparatus of claim 1, wherein the difference between the primary pattern and the secondary pattern comprises a phase shift. ### Claim 15 (depends on 1) The apparatus of claim 1, further comprising a tube configured to have the obtained blood samples accommodated in the tube and detachably coupled to the accommodation unit. ### Claim 16 A tube inserted into an apparatus for measuring a hemoglobin concentration and configured to accommodate blood samples, wherein: the tube is inserted into the apparatus for measuring a hemoglobin concentration which comprises a reference light source unit, a light and heat light source unit, and an image acquisition unit and disposed adjacent to the reference light source unit, the light and heat light source unit, and the image acquisition unit, the tube is provided so that blood samples exposed to a reference beam emitted by the reference light source unit and accommodated in the tube generate a primary pattern, the tube is provided so that blood samples exposed to a light and heat beam emitted by the light and heat light source unit generates a secondary pattern through a light and heat effect, and the tube is configured to accommodate blood samples so that the image acquisition unit calculates a hemoglobin concentration by measuring a difference between the first pattern and the second pattern.