# US 9,518,266 B2 · Maize genes for controlling plant growth and organ size and their use in improving crop plants

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 9,518,266 B2
- Document ID: US9518266B2
- Status: Granted
- Kind: B2
- Application number: 14/028,695
- Filed: 2013-09-17
- Published: 2014-02-06
- Granted: 2016-12-13
- Earliest claimed: 2006-03-31
- Assignees: Pioneer Hi-Bred International, Inc.
- Applicants: Pioneer Hi-Bred International, Inc.
- Inventors: Wesley Bruce; Mei Guo; Rajeev Gupta; Mary Rupe; Carl Simmons
- Web page: https://patentgrep.com/patent/US9518266B2

## CPC

- C12N 15/8261: {with agronomic (input) traits, e.g. crop yield}
- C07K 14/415: from plants
- C12N 15/8273: {for drought, cold, salt resistance}
- C12Q 1/6895: for plants, fungi or algae
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The present invention provides polynucleotides and related polypeptides of the ZmARGOS gene family. The invention provides genomic sequence for the ZmARGOS genes. ZmARGOS is responsible for controlling plant growth, organ size and yield in crop plants.

## Claims

### Claim 1

A method of increasing yield in a maize plant, the method comprising:
a. increasing the expression of a polynucleotide encoding a polypeptide by a heterologous regulatory element in the plant, wherein the polypeptide comprises an amino acid sequence that is at least 95% identical to SEQ ID NO: 11; and
b. increasing the yield of the maize plant compared to a control plant not having increased expression of the polynucleotide.

### Claim 2 (depends on 1)

The method of claim 1, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 11.

### Claim 3

A method of increasing yield of a maize plant under drought stress, the method comprising:
a. increasing the expression of a polynucleotide encoding a polypeptide by a heterologous regulatory element in the plant, wherein the polypeptide comprises an amino acid sequence that is at least 95% identical to SEQ ID NO: 11; and
b. growing the maize plant under drought stress thereby increasing the yield of the plant compared to a control plant not having increased expression of the polynucleotide.

### Claim 4 (depends on 3)

The method of claim 3, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 11.