# US 9,518,252 B2 · Listeria bacteriophage P825 and uses thereof

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- Number: US 9,518,252 B2
- Document ID: US9518252B2
- Status: Granted
- Kind: B2
- Application number: 14/119,811
- Filed: 2012-05-29
- Published: 2015-02-05
- Granted: 2016-12-13
- Earliest claimed: 2011-05-26
- Assignees: Dsm IP Assets B.V.
- Applicants: Holger Grallert; Julia Lorenz; Anna Scherzinger
- Inventors: Holger Grallert; Julia Lorenz; Anna Scherzinger
- Web page: https://patentgrep.com/patent/US9518252B2

## CPC

- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- A01N 63/40: Viruses, e.g. bacteriophages
- A01N 63/50: Isolated enzymes; Isolated proteins (peptides A01N37/46)
- A61K 35/76: Viruses; Subviral particles; Bacteriophages
- C07K 14/005: from viruses
- C07K 16/40: against enzymes
- C12N 9/2462: {Lysozyme (3.2.1.17)}
- C12Q 1/18: Testing for antimicrobial activity of a material
- G01N 33/56911: {Bacteria}
- C12N 2795/00021: Viruses as such, e.g. new isolates, mutants or their genomic sequences
- C12N 2795/00022: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C12N 2795/00031: Uses of virus other than therapeutic or vaccine, e.g. disinfectant
- C12N 2795/00032: Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent
- C12N 2795/00051: Methods of production or purification of viral material
- G01N 2333/32: from Bacillus (G)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The present invention relates to a novel Listeria bacteriophage designated ProCC P825. In particular, the present invention relates to the endolysin PlyP825 encoded by the novel phage ProCC P825 and uses of the novel endolysin PlyP825 for controlling Listeria contamination and infection.

## Claims

### Claim 1

A food product comprising a bacteriophage having a genome
i) comprising the DNA sequence of SEQ ID NO: 7;
ii) having at least 90% or 95% sequence identity with the DNA sequence of SEQ ID NO: 7; or
iii) having at least 90% or 95% sequence identity with the DNA sequence of the genome of bacteriophage ProCC P825 deposited under Accession No. DSM 23783;
wherein the food product is selected from the group consisting of a dairy product, a fruit product, a vegetable product, a meat product, and a fish product.

### Claim 2 (depends on 1)

The food product of claim 1, which is a dairy product.

### Claim 3 (depends on 1)

The food product of claim 1, which is a fruit product.

### Claim 4 (depends on 1)

The food product of claim 1, which is a vegetable product.

### Claim 5 (depends on 1)

The food product of claim 1, which is a meat product.

### Claim 6 (depends on 1)

The food product of claim 1, which is a fish product.

### Claim 7 (depends on 2)

The dairy product of claim 2, which is a pasteurized dairy product.

### Claim 8 (depends on 2)

The dairy product of claim 2, selected from the group consisting of yoghurt, ice cream, cheese and butter.

### Claim 9 (depends on 5)

The meat product of claim 5, selected from the group consisting of pate, hot dog, bologna, salami and cold cuts.

### Claim 10 (depends on 1)

The food product of claim 1, wherein the food product has undergone thermal treatment prior to introducing the bacteriophage.

### Claim 11 (depends on 10)

The food product of claim 10, wherein the thermal treatment is at a temperature of at least 70° C.

### Claim 12 (depends on 2)

The food product of claim 2, wherein the bacteriophage has a genome comprising the DNA sequence of SEQ ID NO: 7.

### Claim 13 (depends on 1)

The food product of claim 1, wherein the bacteriophage has a genome having at least 90% or 95% sequence identity with the DNA sequence of SEQ ID NO: 7.

### Claim 14 (depends on 1)

The food product of claim 1, wherein the bacteriophage has a genome having at least 90% or 95% sequence identity with the DNA sequence of the genome of bacteriophage ProCC P825 deposited under Accession No. DSM 23783.