# US 9,517,468 B2 · Methods and systems for calibration of a positional orientation between a sample container and nozzle tip

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- Number: US 9,517,468 B2
- Document ID: US9517468B2
- Status: Granted
- Kind: B2
- Application number: 14/005,017
- Filed: 2012-03-16
- Published: 2013-12-26
- Granted: 2016-12-13
- Earliest claimed: 2011-03-18
- Assignees: Siemens Healthcare Diagnostics Inc.
- Applicants: Antoine E. Haddad; John Esposito; Joseph Brennan; John Mizzer
- Inventors: Antoine E. Haddad; John Esposito; Joseph Brennan; John Mizzer
- Web page: https://patentgrep.com/patent/US9517468B2

## CPC

- B01L 3/56: {Labware specially adapted for transferring fluids}
- G01N 35/1011: {Control of the position or alignment of the transfer device}
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Disclosed are methods to aid in a calibration of a vertical orientation of a nozzle tip to a sample container in a processing or testing system. The method includes positioning the nozzle over a calibration target at a home height location (HM), moving the nozzle downward a distance (D) until contact with the calibration target is sensed, positioning the nozzle over the sample rack and moving the nozzle downward until contact with a registration location is sensed, imaging the sample rack and calibration target to determine a height (H) between the registration location and calibration target, and calculating a translation ratio (TR) between the height (H) measured in pixel space and the distance (D) measured in machine space. The translation ratio (TR) may be used to drive the nozzle tip to a predetermined depth. A robot calibration system is disclosed, as are other aspects.

## Claims

### Claim 1

A method of calibrating a position of a nozzle tip relative to a sample container, comprising:
providing a nozzle including a nozzle tip, the nozzle operably configured to aspirate a sample fluid from the sample container;
providing a sample rack having a receptacle configured to contain the sample container during the aspirate the sample rack having a registration location;
inserting a calibration target in the receptacle of the sample rack;
positioning the nozzle over the calibration target at a home height location (HM);
moving the nozzle downward from the home height location (HM) a distance (D) until a portion of the calibration target is sensed;
positioning the nozzle over the sample rack;
moving the nozzle downward until a portion of the registration location of the sample rack is sensed to determine a distance (R) from the home height location (HM) to the registration location;
imaging the assembly of the sample rack and the calibration target to determine a height (H) between the registration location and a top of the calibration target in pixel space; and
calculating a translation ratio (TR) between the height (H) measured in the pixel space and the height (M) measured in machine space, wherein (M) is related to distance (R) and height (H);
wherein each of distance (D) and distance (R) is measured in a number of steps (S) of a stepper motor;
the method further comprising: generating the translation ratio (TR) between the number of steps (S) and the height (H); and
using the translation ratio (TR) to drive the nozzle tip to a predetermined drive depth (DD) in a sample container.

### Claim 2 (depends on 1)

The method of calibrating of claim 1, wherein the sensed portion of the calibration target is a bottom of a sample collection tube or small sample cup (SSC).

### Claim 3 (depends on 1)

The method of calibrating of claim 1, wherein the sensed portion of the calibration target is a top of a calibration tool.

### Claim 4 (depends on 1)

The method of calibrating of claim 1, wherein the registration location comprises a planar surface feature on the sample rack.

### Claim 5 (depends on 1)

The method of calibrating of claim 1, wherein the registration location comprises a planar surface feature on the sample rack directly proximate to the calibration target.

### Claim 6

A method of calibrating a position of a nozzle tip relative to a sample container, comprising:
providing a nozzle including a nozzle tip, the nozzle operably configured to aspirate a sample fluid from the sample container;
providing a sample rack having a receptacle configured to contain the sample container during the aspirate the sample rack having a registration location;
inserting a calibration target in the receptacle of the sample rack;
positioning the nozzle over the calibration target at a home height location (HM);
moving the nozzle downward from the home height location (HM) a distance (D) until a portion of the calibration target is sensed;
positioning the nozzle over the sample rack;
moving the nozzle downward until a portion of the registration location of the sample rack is sensed to determine a distance (R) from the home height location (HM) to the registration location;
imaging the assembly of the sample rack and the calibration target to determine a height (H) between the registration location and a top of the calibration target in pixel space; and
calculating a translation ratio (TR) between the height (H) measured in the pixel space and the height (M) measured in machine space, wherein (M) is related to distance (R) and height (H);
the method further comprising, after calibration, imaging a sample container to determine an imaged height of the sample container, and
multiplying the imaged height (IH) by the translation ratio (TR) and a factor to obtain a drive distance to drive the nozzle tip in machine space.

### Claim 7 (depends on 1)

The method of calibrating of claim 1, wherein the calibration target is a calibration tool.

### Claim 8 (depends on 7)

The method of calibrating of claim 7, wherein the calibration tool is electrically conductive, and the distance (D) is determined by sensing a conductive surface of the calibration tool.

### Claim 9 (depends on 8)

The method of calibrating of claim 8, wherein the distance (D) is determined by sensing a load of a drive motor used to drive the nozzle in a Z direction.

### Claim 10

A method of calibrating a position of a nozzle relative to a sample container, comprising:
providing a moveable nozzle having a nozzle tip, the moveable nozzle operably configured to aspirate a specimen;
providing a sample rack having a receptacle and a registration location comprising a planar surface feature;
providing a sample container inserted in the receptacle of the sample rack having a small sample cup (SSC) inserted in the sample container;
positioning the nozzle over the SSC at a home position (HM);
moving the nozzle downward from the home position (HM) until the nozzle tip contacts a register location comprising a bottom surface of the SSC;
determining a distance (D) between the home position (HM) and the bottom surface of the SSC;
imaging the planar surface feature of the sample rack and the top surface of the SSC;
determining a height (H) from the planar surface feature to a top of the SSC, the height (H) determined in pixel space;
and calculating a translation ratio (TR) between the height (H) measured in pixel space and a height (M) measured in machine space, wherein the height (M) is related to the distance (D)
wherein the distance (D) is measured in a number of steps (S) of a stepper motor;
the method further comprising: generating the translation ratio (TR) between the number of steps (S) and the height (H); and
using the translation ratio (TR) to drive the nozzle tip to a predetermined drive depth (DD) in a sample container.