# US 9,517,215 B2 · Dermal matrix and production method thereof having synergistic effects comprising microparticles which provides tissue repair

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- Number: US 9,517,215 B2
- Document ID: US9517215B2
- Status: Granted
- Kind: B2
- Application number: 14/907,563
- Filed: 2014-07-07
- Published: 2016-06-02
- Granted: 2016-12-13
- Earliest claimed: 2013-07-25
- Assignees: T.C. Ege Universitesi
- Applicants: T.C. Ege Universitesi
- Inventors: Kevser Ozgen Ozer; Evren Homan Gokce; Ipek Eroglu; Sakine Tuncay Tanriverdi
- Web page: https://patentgrep.com/patent/US9517215B2

## CPC

- A61K 31/05: Phenols {(cannabinoids A61K31/658)}
- A61L 27/24: Collagen
- A61L 27/26: Mixtures of macromolecular compounds
- A61L 27/54: Biologically active materials, e.g. therapeutic substances {(A61L27/227 takes precedence)}
- A61L 27/56: Porous materials, {e.g. foams or sponges}
- A61L 27/60: Materials for use in artificial skin
- A61P 17/02: for treating wounds, ulcers, burns, scars, keloids, or the like
- A61L 2300/428: Vitamins, e.g. tocopherol, riboflavin
- A61L 2300/622: Microcapsules
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

This invention is related to dermal matrices and production method thereof, having within its structure micro particles having antioxidant agents with synergistic effects, providing speedy repair of the dermal tissue, used in chronic wound treatments, basically comprising the steps of preparing the dermal matrix system (11), forming micro particles (12), combining the micro particles with the dermal matrix system.

## Claims

### Claim 1

A method for producing a dermal matrix system with micro particles, comprising:
preparing the dermal matrix system;
preparing the micro particles; and
combining the micro particles with the dermal matrix system,
wherein the step of preparing the dermal matrix system further comprises,
preparing a collagen solution,
adding laminin into the collagen solution and forming a first mixture,
freezing and storing the first mixture,
lyophilizing the first mixture and forming a lyophilizate,
cross linking the lyophilizate,
wherein the step of preparing the micro particles further comprises,
preparing an aqueous polymer solution, wherein the aqueous polymer solution is prepared by dissolving a hyaluronic acid (HA) in pure water,
preparing a second mixture of phospholipid, an antioxidant agent and alcohol, wherein the second mixture is formed by adding dipalmitoylphosphatidylcholine (DPPC) and resveratrol into the alcohol,
adding the aqueous polymer solution into the second mixture to form a third mixture,
drying the third mixture to obtain the micro particles,
wherein in the step of combining the micro particles with the dermal matrix system, the micro particles are sprayed onto the dermal matrix system.

### Claim 2 (depends on 1)

The method for producing a dermal matrix system with micro particles according to claim 1, wherein the dipalmitoylphosphatidylcholine (DPPC) has a weight of 0.7-0.75 g, the resveratrol has a weight of 0.05-0.1 g and the alcohol has a volume of 350 mL.

### Claim 3 (depends on 1)

The method for producing a dermal matrix system with micro particles according to claim 1, wherein the step of preparing a second mixture is performed at the temperature of 40±1° C.

### Claim 4 (depends on 1)

The method for producing a dermal matrix system with micro particles according to claim 1, wherein in the step of preparing the collagen solution, collagen is added at a concentration of 0.1% into an acetic acid, and the acetic acid has a volume of 0.1M.

### Claim 5 (depends on 1)

The method for producing a dermal matrix system with micro particles according to claim 1, wherein in the step of adding the laminin into the collagen solution and forming the first mixture, the collagen solution has a volume of 2.5 mL, the laminin has a volume of 20 μl, and the first mixture is formed in an ice bath.

### Claim 6 (depends on 1)

The method for producing a dermal matrix system with micro particles according to claim 1, wherein in the step of freezing and storing the first mixture, the first mixture is left to rest for 12 hours at a temperature of −20±1° C.

### Claim 7 (depends on 1)

The method for producing a dermal matrix system with micro particles according to claim 1, wherein the step of lyophilizing the first mixture further comprises lyophilizing the first mixture for 24 hours.

### Claim 8 (depends on 1)

The method for producing a dermal matrix system with micro particles according to claim 1, wherein the step of cross linking the lyophilizate further comprises cross linking the lyophilizate under an ultraviolet light with a wavelength of 254 nm, using an ultraviolet cross linker (UV-cross linker) device.

### Claim 9 (depends on 1)

The method for producing a dermal matrix system with micro particles according to claim 1, wherein in the step of preparing the aqueous polymer solution, the hyaluronic acid (HA) has a weight of 0.2 g, and the pure water has a volume of 150 mL.