# US 9,516,893 B2 · Use of tryptophan rich protein hydrolysates

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- Number: US 9,516,893 B2
- Document ID: US9516893B2
- Status: Granted
- Kind: B2
- Application number: 14/404,878
- Filed: 2013-10-30
- Published: 2015-10-22
- Granted: 2016-12-13
- Earliest claimed: 2012-11-02
- Assignees: Dsm IP Assets B.V.
- Applicants: Dsm IP Assets B.V.
- Inventors: Leigh Gibson; Regina Goralczyk; Hasan Mohajeri; Jonas Wittwer Schegg; Nicolle Goetz; Christina Kalarickal; Simone Koenig-Grillo; Marja Kanning; Damiet Josephina Petronella Cunera Koenders; Kathleen Simons
- Web page: https://patentgrep.com/patent/US9516893B2

## CPC

- A23L 33/175: Amino acids
- A23K 20/147: Polymeric derivatives, e.g. peptides or proteins
- A23L 2/66: Proteins
- A23L 33/18: Peptides; Protein hydrolysates
- A61K 9/0095: {Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches (A61K9/0007 takes precedence; eatable gels or foams A61K9/0056; oral mucosa adhesive forms A61K9/006)}
- A61K 38/47: acting on glycosyl compounds (3.2), e.g. cellulases, lactases
- A61K 47/12: Carboxylic acids; Salts or anhydrides thereof
- A61P 3/02: Nutrients, e.g. vitamins, minerals
- A61P 25/00: Drugs for disorders of the nervous system
- A61P 25/20: Hypnotics; Sedatives
- A61P 25/22: Anxiolytics
- A61P 25/28: for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- C12Y 302/01017: Lysozyme (3.2.1.17)
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A23K: FODDER
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- C12Y: ENZYMES

## Abstract

This invention relates to a method of increasing a feeling of a) energy or b) cognitive functions, selected from the group consisting of: sustained attention, faster reaction times, and information processing; or c) quality of sleep; or d) feelings of well-being and happiness in a healthy adult using a dosage form comprising a low dose of a Trp-containing di- and/or tri-peptide mixture characterized in that the peptide composition is an egg iysozyme hydroiysate with a Trp/L!MAA ratio greater than 0.15 and which provides IQ-to 100 mg Trp per dose. This dosage form can be administered acutely or can be administered multiple times per day for an extended period of time.

## Claims

### Claim 1

An edible composition which imparts a feeling in a healthy adult consumer which is selected from the group consisting of:
a) increased energy;
b) an increase in certain cognitive functions including, sustained attention, faster reaction times, and information processing;
c) increased quality of sleep; or
d) increased feelings of well-being or happiness, wherein the composition is a pharmaceutical, food or food supplement comprising a hen egg lysozyme tryptophan-containing hydrolysate (Trp-containing protein hydrolysate) having a ratio (Trp/LNAA) of tryptophan (Trp) to large neutral amino acid (LNAA) of greater than 0.15, and wherein the dosage of the Trp-containing protein hydrolysate provides 10-100 mg of Trp per day.

### Claim 2 (depends on 1)

The edible composition according to claim 1, wherein the composition is a beverage, a sorbet or a nutritional gel.

### Claim 3 (depends on 1)

The edible composition according to claim 1, wherein the composition is an instant beverage powder which further comprises:
a) a sweetening agent;
a) a flavoring agent; and
a) optional extra nutritional ingredients.

### Claim 4 (depends on 1)

The edible composition according to claim 1, wherein the composition is a beverage which further comprises at least one acid selected from the group consisting of citric acid, malic acid, tartaric acid, fumaric acid, phosphoric acid, acetic acid, succinic acid and adipic acid.

### Claim 5 (depends on 1)

The edible composition according to claim 1, wherein the composition is a sorbet which further comprises:
a) a sweetener or mixture of sweeteners;
b) citric acid and optionally least one additional acid selected from the group consisting of malic acid, tartaric acid, fumaric acid, phosphoric acid, acetic acid, succinic acid and adipic acid; and
c) flavorings.

### Claim 6 (depends on 1)

The edible composition according to claim 1, wherein the composition is a nutritional gel which further comprises:
a) a sweetener or mixture of sweeteners;
b) citric acid or lactic acid, or optionally at least one acid selected from the group consisting of malic acid, tartaric acid, fumaric acid, phosphoric acid, acetic acid; succinic acid, and adipic acid; and
c) flavoring.

### Claim 7 (depends on 1)

A method to impart in a healthy adult consumer a feeling selected from the group consisting of:
a) increased energy
b) an increase in certain cognitive functions, especially sustained attention and faster reaction times, and information processing; or
c) increased quality of sleep; and
d) increased feelings of well-being or happiness, wherein the method comprises administering to a healthy adult consumer an effective amount of the edible food composition according to claim 1.

### Claim 8

A method of increasing:
a) a feeling of energy;
b) cognitive functions selected from the group consisting of sustained attention, faster reaction times, and information processing;
c) quality of sleep; or
d) feelings of well-being and happiness in a healthy adult, wherein the method comprises administering to the healthy adult a pharmaceutical, food or food supplement comprising a hen egg lysozyme tryptophan-containing hydrolysate (Trp-containing protein hydrolysate) having a ratio (Trp/LNAA) of tryptophan (Trp) to large neutral amino acid (LNAA) of greater than 0.15, and wherein the dosage of the Trp-containing protein hydrolysate provides 10-100 mg of Trp per day.

### Claim 9 (depends on 8)

The method according to claim 8, wherein the pharmaceutical, food, or food supplement is a beverage, a sorbet, or a nutritional gel.

### Claim 10

A single dose composition which is a beverage, a beverage powder, a gel, or a sorbet comprising:
a) less than 4000 mg per dosage of a hen egg lysozyme tryptophan-containing hydrolysate (Trp-containing protein hydrolysate) having a ratio (Trp/LNAA) of tryptophan (Trp) to large neutral amino acid (LNAA) of greater than 0.15;
b) a sweetening agent;
c) a flavoring agent; and
d) optional extra nutritional ingredients.

### Claim 11 (depends on 10)

The composition according to claim 10, wherein the amount of the Trp-containing protein hydrolysate per dosage is 2000 mg to 4000 mg.

### Claim 12 (depends on 10)

The composition according to claim 10, wherein the amount of the Trp-containing protein hydrolysate is 1000 mg.

### Claim 13 (depends on 10)

The composition according to claim 10, wherein the amount of the Trp-containing protein hydrolysate is 400-600mg.

### Claim 14 (depends on 10)

The composition according to claim 10, wherein the amount of Trp-containing protein hydrolysate is sufficient to provide at least 10 mg Trp per day but less than 400 mg Trp per day.

### Claim 15 (depends on 10)

The composition according to claim 10, wherein the amount of the Trp-containing hydrolysate is sufficient to provide at least 25 mg Trp per day but less than 350 mg Trp per day.

### Claim 16 (depends on 10)

The composition according to claim 10, wherein the composition is a beverage or beverage powder.

### Claim 17 (depends on 10)

The composition according to claim 10, wherein the composition is a beverage selected from the group consisting of instant beverages, beverage shots, still drinks and carbonated drinks.

### Claim 18 (depends on 10)

The composition according to claim 10, wherein the composition is a beverage powder which further comprises an acid selected from the group consisting of citric acid ,malic acid, tartaric acid, fumaric acid, phosphoric acid, acetic acid, succinic acid, adipic acid and mixtures thereof.

### Claim 19 (depends on 18)

An instant beverage which comprises an admixture of the beverage powder of claim 18 and a liquid.

### Claim 20 (depends on 19)

The instant beverage according to claim 19 wherein the liquid is selected from the group consisting of water, carbonated water, and fruit juice.

### Claim 21 (depends on 10)

The composition according to claim 10, wherein the composition is a gel.

### Claim 22 (depends on 10)

The composition according to claim 10, wherein the composition is a sorbet.