# US 9,516,879 B2 · Chitinous polysaccharide antigen-binding proteins > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 9,516,879 B2 - Document ID: US9516879B2 - Status: Granted - Kind: B2 - Application number: 13/819,330 - Filed: 2011-08-26 - Published: 2013-08-29 - Granted: 2016-12-13 - Earliest claimed: 2010-08-26 - Assignees: Agrosavfe N.V. - Applicants: Peter Verheesen; Chris De Jonghe; Erik Jongedijk - Inventors: Peter Verheesen; Chris De Jonghe; Erik Jongedijk - Web page: https://patentgrep.com/patent/US9516879B2 ## CPC - A01N 37/46: N-acyl derivatives - C07K 16/14: against material from fungi, algea or lichens - C07K 16/16: against material from plants - C07K 16/18: against material from animals or humans - G01N 33/56961: {Plant cells or fungi} - C07K 2317/22: from camelids, e.g. camel, llama or dromedary - C07K 2317/569: Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody® - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Described is an antigen-binding protein, preferably comprising an amino acid sequence that comprises four framework regions and three complementarity-determining regions, wherein the antigen-binding protein is capable of binding a chitinous polysaccharide, and uses thereof. ## Claims ### Claim 1 A single chain antigen-binding protein comprising a VHH sequence, wherein the VHH sequence is able to bind a chitinous polysaccharide. ### Claim 2 (depends on 1) The antigen-binding protein of claim 1, wherein said antigen-binding protein is a monoclonal antigen-binding protein. ### Claim 3 (depends on 1) The antigen-binding protein of claim 1, wherein said chitinous polysaccharide is chitin. ### Claim 4 (depends on 1) The antigen-binding protein of claim 1, wherein said chitinous polysaccharide is comprised in or derived from an arthropod. ### Claim 5 (depends on 1) The antigen-binding protein of claim 1, wherein said chitinous polysaccharide is comprised in or derived from a fungus or a yeast. ### Claim 6 (depends on 1) The antigen-binding protein of claim 1, wherein said antigen-binding protein has an insecticidal activity. ### Claim 7 (depends on 1) The antigen-binding protein of claim 1, wherein said antigen-binding protein has an antifungal activity. ### Claim 8 (depends on 1) The antigen-binding protein of claim 1, wherein said VHH comprises a sequence selected from the group consisting of SEQ ID NO:1 through SEQ ID NO:4. ### Claim 9 (depends on 1) A method of determining the presence and/or concentration of a chitinous polysaccharide in a sample, the method comprising: utilizing the antigen-binding protein of claim 1 to determine the presence and/or concentration of the chitinous polysaccharide in the sample. ### Claim 10 (depends on 1) A method of isolating or purifying a chitinous polysaccharide from a sample, the method comprising: utilizing the antigen-binding protein of claim 1 to isolate or to purify the chitinous polysaccharide from the sample. ### Claim 11 (depends on 1) A method for detecting and/or determining the concentration of one or more chitinous polysaccharides, the method comprising: utilizing at least one antigen-binding protein of claim 1. ### Claim 12 (depends on 1) A targeting agent able to bind a compound to a chitinous polysaccharide, wherein said targeting agent comprises at least one antigen-binding protein of claim 1. ### Claim 13 (depends on 1) A process for producing a transgenic plant, the method comprising: transforming the plant with a nucleic acid molecule comprising a nucleotide encoding the antigen-binding protein of claim 1. ### Claim 14 (depends on 1) The antigen-binding protein of claim 1, further comprising a compound bonded thereto. ### Claim 15 (depends on 14) The antigen-binding protein of claim 14, wherein the compound is an agrochemical composition. ### Claim 16 (depends on 15) The antigen-binding protein of claim 15, wherein the compound has insecticidal activity. ### Claim 17 (depends on 15) The antigen-binding protein of claim 15, wherein the compound has antifungal activity. ### Claim 18 (depends on 1) A method for improving the resistance of a plant against insect pests and/or fungal disease, the method comprising: applying at least once the antigen-binding protein of claim 1 to the plant or to plant parts in an amount that improves the resistance of a plant against insect pests and/or fungal disease. ### Claim 19 (depends on 14) A method of delivering a compound to a chitinous polysaccharide, the method comprising: utilizing the antigen-binding protein of claim 14 to deliver the compound to the chitinous polysaccharide. ### Claim 20 (depends on 19) The method according to claim 19, wherein the chitinous polysaccharide is contained in an insect. ### Claim 21 (depends on 20) The method according to claim 20, wherein the chitinous polysaccharide is contained in a fungus.