# US 9,516,853 B2 · Lettuce variety 83-02 RZ

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- Number: US 9,516,853 B2
- Document ID: US9516853B2
- Status: Granted
- Kind: B2
- Application number: 14/859,950
- Filed: 2015-09-21
- Published: 2016-04-07
- Granted: 2016-12-13
- Earliest claimed: 2014-10-01
- Assignees: Rijk Zwaan Zaadteelt En Zaadhandel B.V.
- Applicants: Rijk Zwaan Zaadteelt En Zaadhandel B.V.
- Inventors: Egbert Carolus Johannes Smits; Clement Michel Bernard Morice
- Web page: https://patentgrep.com/patent/US9516853B2

## CPC

- A01H 6/1472: {Lactuca sativa [lettuce]}
- A01H 5/12: Leaves
- C12N 15/8282: {for fungal resistance}
- C12N 15/8286: {for insect resistance}
- C12Q 1/6895: for plants, fungi or algae
- C12Q 2600/13: Plant traits
- C12Q 2600/156: Polymorphic or mutational markers
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present invention relates to a Lactuca sativa seed designated 83-02 RZ, which may exhibit a combination of traits including resistance to downy mildew (Bremia lactucae) races B1:1-31 and Ca-I to Ca-VIII, resistance to currant-lettuce aphid (Nasonovia ribisnigri), very intensely red deeply lobed leaves, and a loose to moderately firm head. The present invention also relates to a Lactuca sativa plant produced by growing the 83-02 RZ seed. The invention further relates to methods for producing the lettuce cultivar, represented by lettuce variety 83-02 RZ.

## Claims

### Claim 1

A lettuce plant designated 83-02 RZ, representative seed of which having been deposited under NCIMB Accession No. 42311, wherein said plant comprises at least the following combination of traits including resistance to downy mildew (Bremia lactucae) races B1:1-31 and Ca-I to Ca-VIII, resistance to currant-lettuce aphid (Nasonovia ribisnigri), very intensely red deeply lobed leaves, and a loose to moderately firm head.

### Claim 2 (depends on 1)

A seed of the plant of claim 1.

### Claim 3 (depends on 1)

A part of the plant of claim 1, wherein said part of the plant is suitable for sexual reproduction.

### Claim 4 (depends on 3)

The part of the plant as claimed in claim 3, wherein said part comprises a microspore, pollen, ovary, ovule, embryo sac or egg cell.

### Claim 5 (depends on 1)

A part of the plant of claim 1, wherein said part of the plant is suitable for vegetative reproduction.

### Claim 6 (depends on 5)

The part as claimed in claim 5, wherein said part comprises a cutting, root, stem, cell or protoplast.

### Claim 7 (depends on 1)

A tissue culture of regenerable cells or protoplasts from the lettuce plant of claim 1.

### Claim 8 (depends on 7)

The tissue culture as claimed in claim 7, wherein said cells or protoplasts of the tissue culture are derived from a tissue comprising a leaf, pollen, embryo, cotyledon, hypocotyl, meristematic cell, root, root tip, anther, flower, seed or stem.

### Claim 9 (depends on 1)

A method for producing a progeny plant of a lettuce plant of claim 1, comprising crossing the plant of claim 1 with itself or with another lettuce plant, harvesting the resultant seed, and growing said seed.

### Claim 10 (depends on 9)

A progeny plant produced by the method of claim 9, wherein said progeny exhibits a combination of traits including resistance to downy mildew (Bremia lactucae) races B1:1-31 and Ca-I to Ca-VIII, resistance to currant-lettuce aphid (Nasonovia ribisnigri), very intensely red deeply lobed leaves, and a loose to moderately firm head.

### Claim 11 (depends on 9)

A progeny plant produced by the method of claim 9, wherein said progeny plant has all the morphological and physiological characteristics of the lettuce plant designated 83-02 RZ, representative seed of which having been deposited under NCIMB Accession No. 42311.

### Claim 12 (depends on 10)

The progeny plant as claimed in claim 10, wherein said progeny plant is further modified in one or more other characteristics.

### Claim 13 (depends on 12)

The progeny as claimed in claim 12, wherein the modification is effected by mutagenesis.

### Claim 14 (depends on 12)

The progeny as claimed in claim 12, wherein the modification is effected by transformation with a transgene.

### Claim 15 (depends on 1)

A method of producing a hybrid lettuce seed comprising crossing a first parent lettuce plant with a second parent lettuce plant and harvesting the resultant hybrid lettuce seed, wherein said first parent lettuce plant or said second parent lettuce plant is the lettuce plant of claim 1.

### Claim 16 (depends on 15)

A hybrid lettuce seed produced by the method of claim 15.

### Claim 17

A method of producing a lettuce cultivar containing a combination of traits including resistance to downy mildew (Bremia lactucae) races B1:1-31 and Ca-I to Ca-VIII, resistance to currant-lettuce aphid (Nasonovia ribisnigri), very intensely red deeply lobed leaves, and a loose to moderately firm head, representative seed of which having been deposited under NCIMB Accession No. 42311, comprising: crossing a mother lettuce plant with a father lettuce plant to produce a hybrid seed; growing said hybrid seed to produce a hybrid plant; selfing said hybrid plant to produce F2 progeny seed; growing said F2 progeny seed to produce F2-plants; selecting said F2-plants for exhibiting resistance to downy mildew (Bremia lactucae) races B1:1-31 and Ca-I to Ca-VIII, resistance to currant-lettuce aphid (Nasonovia ribisnigri), very intensely red deeply lobed leaves, and a loose to moderately firm head, and, selfing said selected F2-plants to produce F3 progeny seed; growing said F3 progeny seed to produce F3-plants; selecting F3-plants for exhibiting resistance to downy mildew (Bremia lactucae) races B1:1-31 and Ca-I to Ca-VIII, resistance to currant-lettuce aphid (Nasonovia ribisnigri), very intensely red deeply lobed leaves, and a loose to moderately firm head, representative seed of which having been deposited under NCIMB Accession No. 42311, optionally followed by more selfing and selection steps.

### Claim 18 (depends on 17)

A lettuce cultivar produced by the method of claim 17.

### Claim 19 (depends on 1)

A method for producing lettuce leaves as a fresh vegetable comprising packaging leaves of a plant of claim 1.

### Claim 20 (depends on 1)

A method for producing lettuce leaves as a processed food comprising processing leaves of a plant of claim 1.

### Claim 21 (depends on 1)

A container comprising one or more lettuce plants of claim 1 for harvest of leaves.

### Claim 22

A lettuce plant having resistance to downy mildew (Bremia lactucae) races B1:1-31 and Ca-I to Ca-VIII, resistance to currant-lettuce aphid (Nasonovia ribisnigri), very intensely red deeply lobed leaves, and a loose to moderately firm head and other morphological and/or physiological characteristics of a lettuce plant, representative seed of which having been deposited under NCIMB Accession No. 42311.

### Claim 23

A method of introducing a desired trait into a plant of lettuce variety 83-02 RZ comprising: (a) crossing a plant of lettuce variety 83-02 RZ, representative seed of which having been deposited under NCIMB Accession No. 42311, with a second lettuce plant that comprises the desired trait to produce F1 progeny; (b) selecting an F1 progeny that comprises the desired trait; (c) crossing the selected F1 progeny with a plant of lettuce variety 83-02 RZ, to produce backcross progeny and (d) selecting backcross progeny comprising the desired trait and physiological and morphological characteristics of a plant of lettuce variety 83-02 RZ, when grown in the same environmental conditions.

### Claim 24 (depends on 23)

The method of claim 23 further comprising (e) repeating steps (c) and (d) one or more times in succession to produce selected fourth or higher backcross progeny that comprise the desired trait and all of the physiological and morphological characteristics of a plant of lettuce variety 83-02 RZ, when grown in the same environmental conditions.

### Claim 25 (depends on 23)

A lettuce plant produced by the method of claim 23.

### Claim 26

A method for producing a seed of a 83-02 RZ-derived lettuce plant comprising (a) crossing a plant of lettuce variety 83-02 RZ, representative seed of which having been deposited under NCIMB Accession No. 42311, with a second lettuce plant, and (b) whereby seed of a 83-02 RZ-derived lettuce plant forms.

### Claim 27 (depends on 26)

The method of claim 26 further comprising (c) crossing a plant grown from 83-02 RZ-derived lettuce seed with itself or with a second lettuce plant to yield additional 83-02 RZ-derived lettuce seed, (d) growing the additional 83-02 RZ-derived lettuce seed of step (c) to yield additional 83-02 RZ-derived lettuce plants, and (e) repeating the crossing and growing of steps (c) and (d) for an additional 3-10 generations to generate further 83-02 RZ-derived lettuce plants.

### Claim 28 (depends on 27)

The method of claim 27 further comprising selecting at steps b), d), and e), a 83-02 RZ-derived lettuce plant, exhibiting a combination of traits including resistance to downy mildew (Bremia lactucae) races B1:1-31 and Ca-I to Ca-VIII, resistance to currant-lettuce aphid (Nasonovia ribisnigri), very intensely red deeply lobed leaves, and a loose to moderately firm head.

### Claim 29 (depends on 26)

A seed produced by the method of claim 26.

### Claim 30 (depends on 1)

A method of determining the genotype of a plant of lettuce variety 83-02 RZ, representative seed of which has been deposited under NCIMB Accession No. 42311, or a first generation progeny thereof, comprising obtaining a sample of nucleic acids from said plant and comparing said nucleic acids to a sample of nucleic acids obtained from a reference plant, and detecting a plurality of polymorphisms between the two nucleic acid samples, wherein the plurality of polymorphisms are indicative of lettuce variety 83-02 RZ and/or give rise to the expression of any one or more, or all, of the morphological and physiological characteristics of lettuce variety 83-02 RZ as claimed in claim 1.

### Claim 31 (depends on 30)

The method of claim 30 additionally comprising the step of storing the results of detecting the plurality of polymorphisms on a computer readable medium, or transmitting the results of detecting the plurality of polymorphisms.

### Claim 32

A lettuce plant exhibiting a combination of traits including resistance to downy mildew (Bremia lactucae) races B1:1-31 and Ca-I to Ca-VIII, resistance to currant-lettuce aphid (Nasonovia ribisnigri), very intensely red deeply lobed leaves, and a loose to moderately firm head, and having genetic information for so exhibiting the combination of traits, wherein the genetic information is as contained in the plant, representative seed of which having been deposited under NCIMB Accession No. 42311.

### Claim 33 (depends on 1)

The lettuce plant of claim 1, which is a plant grown from seed having been deposited under NCIMB Accession No. 42311.

### Claim 34 (depends on 16)

A hybrid lettuce plant grown from the seed as claimed in claim 16.