# US 9,516,852 B2 · Lettuce named mojito

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- Number: US 9,516,852 B2
- Document ID: US9516852B2
- Status: Granted
- Kind: B2
- Application number: 14/577,243
- Filed: 2014-12-19
- Published: 2015-07-23
- Granted: 2016-12-13
- Earliest claimed: 2014-01-21
- Assignees: Shamrock Seed Company, Inc.
- Applicants: Shamrock Seed Company, Inc.
- Inventors: Larry Knerr
- Web page: https://patentgrep.com/patent/US9516852B2

## CPC

- A01H 6/1472: {Lactuca sativa [lettuce]}
- A01H 5/12: Leaves
- C12N 15/8241: {Phenotypically and genetically modified plants via recombinant DNA technology}
- C12N 15/8271: {for stress resistance, e.g. heavy metal resistance}
- C12N 15/8274: {for herbicide resistance}
- C12N 15/8275: {Glyphosate}
- C12N 15/8277: {Phosphinotricin}
- C12N 15/8278: {Sulfonylurea}
- C12N 15/8279: {for biotic stress resistance, pathogen resistance, disease resistance}
- C12N 15/8286: {for insect resistance}
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Novel lettuce cultivar, designated MOJITO, is disclosed. The invention relates to the seeds of lettuce cultivar MOJITO, to the plants of lettuce line MOJITO and to methods for producing a lettuce plant by crossing the cultivar MOJITO with itself or another lettuce line, respectively. The invention further relates to methods for producing a lettuce plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other lettuce lines derived from the cultivar MOJITO.

## Claims

### Claim 1

A seed of lettuce cultivar designated MOJITO, wherein a representative sample of seed of said cultivar has been deposited under NCIMB No. 42581.

### Claim 2 (depends on 1)

A lettuce plant, or a part thereof, produced by growing the seed of claim 1.

### Claim 3 (depends on 2)

A tissue culture of regenerable cells produced from the plant of claim 2 wherein said cells of the tissue culture are produced from a plant part selected from the group consisting of embryos, meristematic cells, leaves, pollen, root, root tips, stems, anther, pistils, axillary buds, flowers and seeds.

### Claim 4 (depends on 3)

A lettuce plant regenerated from the tissue culture of claim 3, said plant having the morphological and physiological characteristics of lettuce cultivar MOJITO, wherein a representative sample of seed has been deposited under NCIMB No. 42581.

### Claim 5 (depends on 2)

A method for producing a lettuce seed comprising crossing a first parent lettuce plant with a second parent lettuce plant and harvesting the resultant hybrid lettuce seed, wherein said first parent lettuce plant or second parent lettuce plant is the lettuce plant of claim 2.

### Claim 6 (depends on 5)

A hybrid lettuce seed produced by the method of claim 5.

### Claim 7 (depends on 2)

A method for producing an herbicide resistant plant comprising transforming the lettuce plant of claim 2 with a transgene that confers herbicide resistance to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine, and benzonitrile.

### Claim 8 (depends on 7)

An herbicide resistant lettuce plant, or a part thereof, produced by the method of claim 7.

### Claim 9 (depends on 2)

A method for producing an insect resistant lettuce plant comprising transforming the lettuce plant of claim 2 with a transgene that confers insect resistance.

### Claim 10 (depends on 9)

An insect resistant lettuce plant, or a part thereof, produced by the method of claim 9.

### Claim 11 (depends on 2)

A method for producing a disease resistant lettuce plant comprising transforming the lettuce plant of claim 2 with a transgene that confers disease resistance.

### Claim 12 (depends on 11)

A disease resistant lettuce plant, or a part thereof, produced by the method of claim 11.

### Claim 13 (depends on 2)

A method of producing a lettuce plant derived from the lettuce MOJITO, the method comprising the steps of:
(a) preparing a progeny plant derived from MOJITO by crossing the plant of claim 2 with a second lettuce plant;
(b) crossing the progeny plant with itself or a second lettuce plant to produce a seed of a progeny plant of a subsequent generation;
(c) growing a progeny plant of a subsequent generation from said seed and crossing the progeny plant of a subsequent generation with itself or a second lettuce plant; and
(d) repeating step b) or c) for at least 1 more generation to produce a lettuce plant derived from the lettuce MOJITO.

### Claim 14 (depends on 1)

A lettuce head, produced by growing the seed of claim 1.

### Claim 15

A lettuce plant, or a part thereof, having all the physiological and morphological characteristics of lettuce cultivar MOJITO listed in Table 4.

### Claim 16

A lettuce plant, or a part thereof, having the physiological and morphological characteristics of lettuce cultivar MOJITO, wherein a representative sample of seed of said cultivar has been deposited under NCIMB No. 42581.

### Claim 17 (depends on 16)

A method for producing lettuce cultivar MOJITO seed comprising crossing a first parent lettuce plant with a second parent lettuce plant and harvesting the resultant lettuce seed, wherein both said first and second lettuce plants are the lettuce plant of claim 16.

### Claim 18

A method of introducing a desired trait into lettuce cultivar MOJITO comprising:
(a) crossing a lettuce cultivar MOJITO plant grown from lettuce cultivar MOJITO seed, wherein a representative sample of seed has been deposited under NCIMB No. 42581, with another lettuce plant that comprises a desired trait to produce F1 progeny plants, wherein the desired trait is selected from the group consisting of insect resistance, disease resistance, water stress tolerance, heat tolerance, improved shelf life, delayed senescence, and improved nutritional quality;
(b) selecting one or more progeny plants that have the desired trait to produce selected progeny plants;
(c) crossing the selected progeny plants with the lettuce cultivar MOJITO plants to produce backcross progeny plants;
(d) selecting for backcross progeny plants that have the desired trait and physiological and morphological characteristics of lettuce cultivar MOJITO listed in Table 4 to produce selected backcross progeny plants; and
(e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise the desired trait and the physiological and morphological characteristics of lettuce cultivar MOJITO listed in Table 4.

### Claim 19 (depends on 18)

A lettuce plant produced by the method of claim 18, wherein the plant has the desired trait and the physiological and morphological characteristics of lettuce cultivar MOJITO listed in Table 4 when grown under the same environmental conditions.

### Claim 20 (depends on 19)

The lettuce plant of claim 19, wherein the desired trait is herbicide resistance and the resistance is conferred to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine, and benzonitrile.

### Claim 21 (depends on 19)

The lettuce plant of claim 19, wherein the desired trait is insect resistance and the insect resistance is conferred by a transgene encoding a Bacillus thuringiensis endotoxin.

### Claim 22 (depends on 19)

The lettuce plant of claim 19, wherein the desired trait is selected from the group consisting of insect resistance, disease resistance, water stress tolerance, heat tolerance, improved shelf life, delayed senesence, and improved nutritional quality.