# US 9,516,851 B2 · Onion variety NUN 03010 ON

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- Number: US 9,516,851 B2
- Document ID: US9516851B2
- Status: Granted
- Kind: B2
- Application number: 13/782,033
- Filed: 2013-03-01
- Published: 2013-07-11
- Granted: 2016-12-13
- Earliest claimed: 2013-03-01
- Assignees: Nunhems B.V.
- Applicants: Nunhems B.V.
- Inventors: Michael Bowman
- Web page: https://patentgrep.com/patent/US9516851B2

## CPC

- A01H 6/045: {Allium cepa [onion]}
- A01H 5/04: Stems
- A01H 5/12: Leaves
- C12N 15/8241: {Phenotypically and genetically modified plants via recombinant DNA technology}
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The invention relates to the field of Allium in particular to a new variety of Allium cepa L. designated NUN 3010 ON plants, seeds and bulbs thereof as well as plant breeding methods involving NUN 3010 ON.

## Claims

### Claim 1

An onion plant of variety NUN 3010, a representative sample of seed of said variety having been deposited under ATCC Accession Number 42662.

### Claim 2

A seed of variety NUN 3010, a representative sample of seed of said variety having been deposited under ATCC Accession Number 42662.

### Claim 3 (depends on 1)

A plant part of the plant of claim 1 wherein said plant part comprises the same genetic makeup as the plant of claim 1.

### Claim 4 (depends on 3)

The plant part of claim 3, further defined as a leaf, pollen, cutting, flower, pollen, meristem, bud, root, root tip, an ovule, a bulb, bulb-scales, or a cell.

### Claim 5 (depends on 3)

The plant part of claim 3, further defined as a bulb.

### Claim 6 (depends on 1)

An onion plant, or a part thereof, which has all the physiological and morphological characteristics of Table 1 of the plant of claim 1 when grown under the same conditions.

### Claim 7 (depends on 1)

A tissue culture of regenerable cells of the plant of claim 1.

### Claim 8 (depends on 7)

The tissue culture according to claim 7, comprising cells or protoplasts from a plant part selected from the group consisting of embryos, meristems, cotyledons, pollen, leaves, anthers, roots, root tips, pistil, flower, seed and bulbs.

### Claim 9 (depends on 1, 7)

An onion plant regenerated from the tissue culture of claim 7, comprising all the physiological and morphological characteristics of the plant of claim 1 as provided in Table 1.

### Claim 10 (depends on 1)

A method of vegetatively propagating the plant of claim 1 comprising the steps of:
(a) collecting tissue capable of being propagated from a plant according to claim 1;
(b) cultivating said tissue to obtain proliferated shoots; and
(c) rooting said proliferated shoots to obtain rooted plantlets.

### Claim 11 (depends on 10)

The method of claim 10, further comprising growing plants from said rooted plantlets.

### Claim 12 (depends on 1)

A method of producing an onion plant, said method comprising crossing the plant of claim 1 with a second onion plant one or more times, and selecting progeny from said crossing.

### Claim 13

A method of introducing a desired trait into an onion variety comprising:
(a) crossing a plant of variety NUN 3010, a representative sample of seed of said variety having been deposited under ATCC Accession Number 42662 with a second onion plant that comprises a desired trait to produce F1 progeny;
(b) selecting an F1 progeny that comprises the desired trait;
(c) optionally selfing the F1 progeny one or more times to produce F2, F3 or further generation selfing progeny;
(d) crossing the selected F1 progeny or the selfing progeny with a plant of variety NUN 3010 to produce backcross progeny;
(e) selecting backcross progeny comprising the desired trait and essentially all the physiological and morphological characteristic of onion variety NUN 3010; and
optionally
(f) repeating steps (d) and (e) one or more times in succession to produce selected fourth or higher backcross progeny that comprise the desired trait.

### Claim 14

A method of producing a plant comprising an added desired trait, the method comprising introducing a transgene conferring the desired trait into a plant of onion variety NUN 3010.

### Claim 15

A method for producing a seed of a variety derived from NUN 3010 comprising the steps of:
(a) crossing an onion plant of variety NUN 3010 with a second onion plant; and
(b) allowing seed of a variety NUN 3010-derived onion plant to form.

### Claim 16 (depends on 14)

The method of claim 14 further comprising the steps of:
(c) crossing a plant grown from said variety NUN 3010-derived onion seed with itself or a second onion plant to yield additional variety NUN 3010-derived onion seed;
(d) growing said additional variety NUN 3010-derived onion seed of step (c) to yield additional variety NUN 3010-derived onion plants; and optionally
(e) repeating the crossing and growing steps of (c) and (d) to generate further variety NUN 3010-derived onion plants.

### Claim 17 (depends on 14)

The method of claim 14, wherein the second onion plant is of an inbred onion variety.

### Claim 18 (depends on 1)

A method of producing an onion bulb comprising:
(a) obtaining a plant according to claim 1, wherein the plant has been cultivated to maturity; and
(b) collecting a bulb from said plant.

### Claim 19 (depends on 1)

A food or feed product comprising a bulb or a bulb-part of the plant of claim 1.