# US 9,516,850 B2 · Wheat cultivar HY 271-SRW and compositions and methods of using the same

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- Number: US 9,516,850 B2
- Document ID: US9516850B2
- Status: Granted
- Kind: B2
- Application number: 14/706,481
- Filed: 2015-05-07
- Published: 2015-11-19
- Granted: 2016-12-13
- Earliest claimed: 2014-05-14
- Assignees: Agrigenetics, Inc.
- Applicants: Agrigenetics, Inc.
- Inventors: Mark J. Etienne
- Web page: https://patentgrep.com/patent/US9516850B2

## CPC

- A01H 6/4678: {Triticum sp. [wheat]}
- A01H 5/10: Seeds
- C12N 15/8241: {Phenotypically and genetically modified plants via recombinant DNA technology}
- C12N 15/8245: {involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis}
- C12N 15/8247: {involving modified lipid metabolism, e.g. seed oil composition}
- C12N 15/8251: {Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis}
- C12N 15/8271: {for stress resistance, e.g. heavy metal resistance}
- C12N 15/8274: {for herbicide resistance}
- C12N 15/8279: {for biotic stress resistance, pathogen resistance, disease resistance}
- C12N 15/8286: {for insect resistance}
- C12N 15/8289: {Male sterility}
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A wheat cultivar designated HY 271-SRW is disclosed. The invention relates to the seeds and plants of wheat cultivar HY 271-SRW and to methods for producing wheat seeds and plants by crossing wheat cultivar HY 271-SRW with itself or another wheat cultivar or wheat plant not designated a cultivar. The invention also relates to methods for producing seeds and plants of wheat cultivar HY 271-SRW containing in its genetic material one or more transgenes and to the transgenic wheat plants and plant parts produced by those methods. The invention also relates to methods for producing seeds and plants by mutagenesis of wheat cultivar HY 271-SRW. The invention also relates to hybrid wheat seeds and plants produced by crossing wheat cultivar HY 271-SRW with another wheat cultivar.

## Claims

### Claim 1

A seed of soft red winter wheat cultivar designated HY 271-SRW, representative seed of said cultivar having been deposited under ATCC Accession No: PTA-122500.

### Claim 2 (depends on 1)

A wheat plant, or part thereof, produced by growing the seed of claim 1.

### Claim 3 (depends on 2)

A tissue culture of regenerable cells produced from the plant of claim 2.

### Claim 4 (depends on 3)

A protoplast produced from the tissue culture of claim 3.

### Claim 5 (depends on 3)

The tissue culture of claim 3, wherein the cells of the tissue culture are from a plant part selected from the group consisting of meristematic tissue, anthers, leaves, embryos, pollen, kernel, head, stem, root, root tip, ovule, and flower.

### Claim 6 (depends on 3)

A wheat plant regenerated from the tissue culture cells of claim 3, said plant having all the morphological and physiological characteristics of wheat cultivar HY 271-SRW.

### Claim 7 (depends on 2)

Grain harvested from the plant of claim 2.

### Claim 8 (depends on 7)

A grain product produced from min harvested from claim 7.

### Claim 9 (depends on 2)

A method for producing an F1 wheat seed, comprising crossing the plant of claim 2, with a different wheat plant and harvesting the resulting F1 wheat seed.

### Claim 10 (depends on 2)

A method of producing a male-sterile wheat plant comprising transforming the wheat plant of claim 2, with a nucleic acid molecule that confers male sterility.

### Claim 11 (depends on 2)

A method of producing an herbicide-resistant, insect-resistant, or abiotic-stress-tolerant wheat plant comprising transforming the wheat plant of claim 2, with a transgene that confers herbicide resistance, insect resistance, disease or abiotic stress tolerance.

### Claim 12 (depends on 11)

An herbicide-resistant wheat plant produced by the method of claim 11.

### Claim 13 (depends on 11)

An insect-resistant wheat plant produced by the method of claim 11.

### Claim 14 (depends on 11)

An abiotic-stress-tolerant wheat plant produced by the method of claim 11.

### Claim 15 (depends on 11)

A wheat plant produced by the method of claim 11, wherein the transgene encodes a Bacillus thuringiensis endotoxin.

### Claim 16 (depends on 2)

A method of producing a wheat plant with modified fatty acid metabolism, modified protein metabolism or modified carbohydrate metabolism comprising transforming the wheat plant of claim 2 with a transgene encoding a polypeptide selected from the group consisting of modified glutenins, gliadins, stearyl-ACP-desaturase, fructosyltransferase, levansucrase, alpha-amylase, invertase, soluble starch synthase(s), starch branching enzyme(s), and starch de-branching enzyme(s).

### Claim 17

A method of introducing a desired trait into wheat cultivar HY 271-SRW comprising: (a) crossing HY 271-SRW plants grown from HY 271-SRW seed, representative seed of which has been deposited under ATCC Accession No: PTA-122500, with plants of another wheat line that comprise a desired trait to produce F1 progeny plants, wherein the desired trait is selected from the group consisting of male sterility, herbicide resistance, insect resistance, disease resistance and waxy starch; (b) selecting F1 progeny plants that have the desired trait to produce selected F1 progeny plants; (c) crossing the selected progeny plants with the HY 271-SRW plants to produce backcross progeny plants; (d) selecting for backcross progeny plants that have the desired trait and physiological and morphological characteristics of wheat cultivar HY 271-SRW to produce selected backcross progeny plants; and (e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise the desired trait and all of the physiological and morphological characteristics of wheat cultivar HY 271-SRW.

### Claim 18 (depends on 17)

A wheat plant produced by the method of claim 17, wherein the plant has the desired trait and all of the physiological and morphological characteristics of wheat cultivar HY 271-SRW.

### Claim 19 (depends on 17)

A disease resistant wheat plant produced by the method of claim 17.
