# US 9,516,843 B2 · Canola variety 45H33

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- Number: US 9,516,843 B2
- Document ID: US9516843B2
- Status: Granted
- Kind: B2
- Application number: 14/633,182
- Filed: 2015-02-27
- Published: 2016-09-01
- Granted: 2016-12-13
- Earliest claimed: 2015-02-27
- Assignees: Pioneer Hi-Bred International, Inc.
- Applicants: Pioneer Hi-Bred International Inc
- Inventors: Jayantilal Devabhai Patel; Igor Falak
- Web page: https://patentgrep.com/patent/US9516843B2

## CPC

- A01H 6/202: {Brassica napus [canola]}
- A01H 5/10: Seeds
- C12N 15/8243: {involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine}
- C12N 15/8245: {involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis}
- C12N 15/8247: {involving modified lipid metabolism, e.g. seed oil composition}
- C12N 15/8251: {Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis}
- C12N 15/8271: {for stress resistance, e.g. heavy metal resistance}
- C12N 15/8274: {for herbicide resistance}
- C12N 15/8279: {for biotic stress resistance, pathogen resistance, disease resistance}
- C12N 15/8286: {for insect resistance}
- C12N 15/8289: {Male sterility}
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Provided is a canola variety designated 45H33 and seed, plants and plant parts thereof produced from a cross of inbred varieties. Methods for producing a canola variety comprise crossing canola variety 45H33 with another canola plant. Methods for producing a canola plant containing in its genetic material one or more traits introgressed into 45H33 through backcross conversion and/or transformation, and to the canola seed, plant and plant part produced thereby are described. Canola variety 45H33, the seed, the plant produced from the seed, plant parts and variants, mutants, and minor modifications of canola variety 45H33 are disclosed.

## Claims

### Claim 1

A canola variety 45H33, representative seed of the variety having been deposited under ATCC Accession Number PTA-122029.

### Claim 2 (depends on 1)

A seed of the canola variety of claim 1.

### Claim 3 (depends on 2)

The seed of claim 2, further comprising a seed treatment.

### Claim 4 (depends on 3)

The seed of claim 3, wherein the treatment comprises a fungicide or insecticide.

### Claim 5 (depends on 2)

A method comprising cleaning the seed of claim 2.

### Claim 6 (depends on 2)

A method of producing a canola seed, the method comprising planting the seed of claim 2 to produce a subsequent generation of seed; harvesting the subsequent generation of seed; and planting the subsequent generation of seed.

### Claim 7 (depends on 2)

The seed of claim 2, further comprising a trait introduced by backcrossing or genetic transformation.

### Claim 8 (depends on 7)

The seed of claim 7, wherein the trait is selected from the group consisting of male sterility, a site for site-specific recombination, abiotic stress tolerance, altered phosphate, altered antioxidants, altered fatty acids, altered essential amino acids, altered carbohydrates, herbicide resistance, insect resistance and disease resistance.

### Claim 9 (depends on 7)

The seed of claim 7, further comprising a seed treatment.

### Claim 10 (depends on 9)

The seed of claim 9, wherein the treatment comprises a fungicide or insecticide.

### Claim 11 (depends on 7)

A method comprising cleaning the seed of claim 7.

### Claim 12 (depends on 1)

A plant or plant part of the canola variety of claim 1.

### Claim 13 (depends on 12)

A method of producing a second canola plant, the method comprising applying plant breeding techniques to the plant or plant part of claim 12, thereby producing the second canola plant.

### Claim 14 (depends on 12)

A method for producing a canola plant derived from canola variety 45H33, the method comprising:
(a) crossing the plant of claim 12 with itself or a second plant to produce progeny seed;
(b) growing the progeny seed to produce a progeny plant and crossing the progeny plant with itself or a different plant to produce further progeny seed; and
(c) repeating step (b) for at least one additional generation to produce a canola plant derived from the variety 45H33.

### Claim 15 (depends on 12)

A method for producing a second canola plant or plant part, the method comprising doubling haploid seed generated from the plant of claim 12, thereby producing the second canola plant or plant part.

### Claim 16 (depends on 12)

The plant or plant part of claim 12, further comprising a trait introduced by backcrossing or genetic transformation.

### Claim 17 (depends on 16)

The plant or plant part of claim 16, wherein the trait is selected from the group consisting of male sterility, a site for site-specific recombination, abiotic stress tolerance, altered phosphate, altered antioxidants, altered fatty acids, altered essential amino acids, altered carbohydrates, herbicide resistance, insect resistance and disease resistance.

### Claim 18 (depends on 16)

A method for producing a second canola plant or plant part, the method comprising applying plant breeding techniques to the plant or plant part of claim 16, thereby producing the second canola plant.

### Claim 19 (depends on 16)

A method for producing a canola plant derived from canola variety 45H33, the method comprising:
(a) crossing the plant of claim 16 with itself or a second plant to produce progeny seed;
(b) growing the progeny seed to produce a progeny plant and crossing the progeny plant with itself or a different plant to produce further progeny seed; and
(c) repeating step (b) for at least one additional generation to produce a canola plant derived from the variety 45H33 and further comprising the trait.

### Claim 20 (depends on 16)

A method for producing a second canola plant or plant part, the method comprising doubling haploid seed generated from the plant of claim 16, thereby producing the second canola plant or plant part.