# US 9,516,823 B2 · Plasmodiophora brassicae-resistant Brassica plant, seeds and plant parts thereof and methods for obtaining the same

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- Number: US 9,516,823 B2
- Document ID: US9516823B2
- Status: Granted
- Kind: B2
- Application number: 13/989,977
- Filed: 2011-11-28
- Published: 2013-11-14
- Granted: 2016-12-13
- Earliest claimed: 2010-11-29
- Assignees: Bejo Zaden B.V.
- Applicants: Laurentius Petrus Nicolaas Martinus Kroon; Albertus Johannes Maria Schrijver; Roelof Marinus Veenstra; Klaas Biersteker
- Inventors: Laurentius Petrus Nicolaas Martinus Kroon; Albertus Johannes Maria Schrijver; Roelof Marinus Veenstra; Klaas Biersteker
- Web page: https://patentgrep.com/patent/US9516823B2

## CPC

- A01H 6/203: {Brassica oleraceae, e.g. broccoli or kohlrabi}
- C12Q 1/6895: for plants, fungi or algae
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present invention relates to a Plasmodiophora brassicae-resistant Brassica plant. The present invention relates more specifically to a Plasmodiophorabrassicae-resistant Brassica plant comprising in its genome two or more genetic factors imparting resistance, where in the presence of the genetic factors imparting resistance can be determined by the presence of a Quantitative Trait Locus (QTL1) and a Quantitative Trait Locus (QTL3) and/or a Quantitative Trait Locus 5 (QTL5).

## Claims

### Claim 1

A Plasmodiophora brassicae-resistant hybrid Brassica oleracea plant comprising in its genome three or more genetic factors imparting resistance, wherein the Plasmodiophora brassicae-resistance is from a plant with deposit number NCIMB 41685; wherein the genome includes a Quantitative Trait Locus 1 (QTL1) and a Quantitative Trait Locus 3 (QTL3) and a Quantitative Trait Locus 5 (QTL5),
wherein QTL1 is characterized by a Random Amplified Microsatellite Polymorphism (RAMP) marker comprising a fragment of 294 bp with primer combination SEQ ID NOs:1 and 7;
QTL3 is characterized by a RAMP marker comprising a fragment of 159 bp with primer combination SEQ ID NOs:9 and 7; and
QTL5 is characterized by a RAMP marker comprising a fragment of 276 bp with primer combination SEQ ID NOs:16 and 7.

### Claim 2 (depends on 1)

The plant as claimed in claim 1, wherein the QTL1, QTL3, and QTL5 are characterized by two or more RAMP markers.

### Claim 3 (depends on 1)

The plant as claimed in claim 1, wherein the QTL1, QTL3, and QTL5 are characterized by three or more RAMP markers.

### Claim 4 (depends on 1)

The plant as claimed in claim 1, wherein the QTL1, QTL3, and QTL5 are characterized by four or more RAMP markers.

### Claim 5 (depends on 1)

The plant as claimed in claim 1, wherein the plant is a Brassica plant selected from the group consisting of B. oleracea convar. botrytis var. botrytis (cauliflower, romanesco broccoli), B. oleracea convar. botrytis var. cymosa (broccoli), B. oleracea convar. botrytis var. asparagoides (sprouting broccoli), B. oleracea convar. oleracea var. gemnifera (Brussels sprout), B. oleracea convar. capitata var. alba (white cabbage, oxheart cabbage), B. oleracea convar. capitata var. rubra (red cabbage), B. oleracea convar. capitata var. sabauda (savoy cabbage), B. oleracea convar. acephala var. sabellica (curly cale cabbage), B. oleracea convar. acephela var. gongyloides (turnip cabbage) and B. oleracea var. tronchuda syn. costata (Portuguese cabbage).

### Claim 6 (depends on 1)

The plant as claimed in claim 1, wherein the plant is derived from a plant with the deposit number NCIMB 41685.

### Claim 7 (depends on 1)

Seeds, fruits and/or plant parts of a Brassica plant as claimed in claim 1, which comprise QTL1, QTL3, and QTL5 as defined in claim 1.