Cell-based production of nonulosonates
AssigneesNational Research Council of Canada, University of Ottawa
InventorsChristopher N. Boddy, Susan M. Logan, Benjamin R. Lundgren, Ian C. Schoenhofen, Dennis M. Whitfield
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Abstract
The present invention relates to the cell-based production of bacterial nonulosonates and their biosynthetic precursors. Specifically, the present invention provides recombinant cells for the production of pseudaminic acid, legionaminic acid, UDP-2,4-diacetamido-2,4,6-trideoxy-□-L-altropyranose, and UDP-2,4-diacetamido-2,4,6-trideoxy-□-D-glucopyranose. Methods for producing the sugars are also provided.
Claims11
A recombinant cell for the production of legionarninic acid, comprising an inactivated GicNAc-6-P deacetylase gene, a gene encoding a PglF enzyme, a gene encoding a PglE enzyme, a gene encoding a PglD enzyme, a gene encoding a Legl enzyme, a gene encoding a LegG enzyme, a gene encoding a GlcNAc-6-P mutase, and a gene encoding a GlcNAc-1-P uridyltransferase.
A recombinant cell for the production of UDP-2,4-diacetamido-2,4,6-trideoxy-α-D-glucopyranose (UDP-BacdiNAc), comprising an inactivated GlcNAc-6-P deacetylase gene, a gene encoding a PglF enzyme, a gene encoding a PglE enzyme, a gene encoding a PglD enzyme, a gene encoding a GlcNAc-6-P mutase, and a gene encoding a GlcNAc-1-P uridyltransferase.
Dependencies are inferred from the claim text.