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AssigneeCornell Research Foundation, Inc.
InventorsMatthew Delisa, Cassandra Guarino, Thomas Mansell, Adam Fisher
Estimating the term…
The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyltransferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format. A glycosylated antibody comprising an Fv portion which recognizes and binds to a native antigen and an Fc portion which is glycosylated at a conserved asparagine residue is also disclosed.
A recombinant prokaryotic host cell expressing one or more eukaryotic UDP-GlcNAc transferase enzymes, one or more eukaryotic mannosyltransferase enzymes, and a prokaryotic oligosaccharyltransferase enzyme capable of transferring a eukaryotic glycan to an N-glycosylation acceptor site of a protein, said acceptor site comprising N-X-S/T.
Dependencies are inferred from the claim text.