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US 9,506,089 B2Granted
Microorganism having novel acrylic acid synthesis pathway and method of producing acrylic acid by using the microorganism
AssigneeSamsung Electronics Co., Ltd.
InventorsHunsu Chu, Jinho Ahn, Taewook Nam, Jiae Yun, Insuk Choi, Yeoju Song, Jinsuk Lee
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Abstract
A microorganism capable of producing an acrylic acid (AA), wherein activities of a pathway producing AA through conversions of 3-HP to 3-HP-CoA and 3-HP-CoA to AA-CoA in the microorganisms are increased; as well as a method of producing the microorganism and a method of producing an acrylic acid by using the same.
Claims13
A genetically engineered microorganism that produces acrylate, wherein the genetically engineered microorganism comprises:
- an exogenous polynucleotide encoding a coenzyme A (CoA) transferase catalyzing conversion of 3-hydroxypropionic acid (3-HP) to 3-hydroxypropionyl-CoA (3-HP-CoA) and belonging to EC 2.8.3.8, EC 3.1.2-, or EC 6.2.1.7,
- an exogenous polynucleotide encoding a 3-HP-CoA dehydratase catalyzing conversion of 3-HP-CoA to acrylyl-CoA and belonging to EC 4.2.1,
- an exogenous polynucleotide encoding a 3-HP-CoA hydrolase or a 3-hydroxyisobutyryl-CoA hydrolase belonging to EC 3.1.2,
- a polynucleotide encoding an enzyme catalyzing the conversion of glycerol to 3-propionic aldehyde (3-HPA), and
- a polynucleotide encoding an enzyme catalyzing the conversion of 3-HPA to 3-HP, and
- wherein the genetically engineered microorganism has increased expression of the exogenous polynucleotides as compared to a microorganism of the same type that does not comprise the exogenous polynucleotides,
- wherein the genetically engineered microorganism is an Escherichia, and
- wherein the Escherichia produces 3-HP.
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An expression vector comprising:
- a polynucleotide encoding a coenzyme A (CoA) transferase catalyzing conversion of 3-hydroxypropionic acid (3-HP) to 3-hydroxypropionyl-CoA (3-HP-CoA) and belonging to EC 2.8.3.8, EC 3.1.2-, or EC 6.2.1.7,
- a polynucleotide encoding a 3-HP-CoA dehydratase catalyzing conversion of 3-HP-CoA to acrylyl-CoA and belonging to EC 4.2.1,
- a polynucleotide encoding a 3-HP-CoA hydrolase or a 3-hydroxyisobutyryl-CoA hydrolase belonging to EC 3.1.2, and
- a polynucleotide that is heterologous to the polynucleotide encoding the CoA transferase, the polynucleotide encoding the 3-HP-CoA dehydratase, the polynucleotide encoding the 3-HP-CoA hydrolase, or the 3-hydroxyisobutyryl-CoA hydrolase.
1 more element
Dependencies are inferred from the claim text.