Method of packing chromatographic columns
AssigneePurdue Research Foundation
InventorsMary Jean Wirth, Sampath Ranasinghe Kodithuwakkuge, Charu Yerneni, Robert Birdsall
Life of this patent
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Abstract
Improved methods for packing a chromatographic column and columns packed by these methods are provided. The methods include introducing a packing material into a chromatographic column, while simultaneously ultrasonicating and applying pressure to the packing material to form a packed column with radial homogeneity.
Claims24
A method for packing a chromatographic column comprising silica particles, the method comprising the steps of
- a) introducing the silica particles into the chromatographic column, wherein the chromatographic column has a length of 2 to 10 cm and an inner diameter of 25 μm to 5 cm, and the silica particles have a diameter of 100 nm to 900 nm;
- b) ultrasonicating the chromatographic column;
- and
- c) applying pressure to the silica particles,
- wherein steps a)-c) are performed simultaneously and wherein a packed chromatographic column with an eddy diffusion term of less than 1 μM is formed, wherein the silica particles are uniformly packed and exhibit no cracks or uninterrupted contact between the inner surface of the column and the silica particles, and wherein the packed chromatographic column is suitable for HPLC, UHPLC, or FPLC.
A packed chromatographic column comprising:
- (i) a chromatographic column having a length of 2 to 10 cm and an inner diameter of 25 μm to 5 cm, and
- (ii) silica particles inside the chromatographic column, wherein the silica particles have a diameter of 100 nm to 900 nm,
- the packed chromatographic column prepared by the steps of
- a) introducing the silica particles into the chromatographic column;
- b) ultrasonicating the chromatographic column;
- and
- C) applying pressure to the silica particles,
- wherein steps a)-c) are performed simultaneously,
- wherein the packed chromatographic column has an eddy diffusion term of less than 1 μm;
- wherein the silica particles are uniformly packed and exhibit no cracks or uninterrupted contact between the inner surface of the chromatographic column and the silica particles;
- and
- wherein the packed chromatographic column is suitable for HPLC, UHPLC or FPLC.
A packed chromatographic column comprising silica particles inside a column, wherein the silica particles have a diameter of 100 nm to 150 nm, the packed chromatographic column prepared by the steps of
- a) introducing the silica particles into the column;
- b) ultrasonicating the column;
- and
- c) applying pressure to the silica particles,
- wherein steps a)-c) are performed simultaneously,
- wherein the packed chromatographic column has an eddy diffusion term of less than 1 μm;
- wherein the silica particles are uniformly packed and exhibit no cracks or uninterrupted contact between the inner surface of the column and the silica particles, and
- wherein the packed chromatographic column is suitable for HPLC, UHPLC or FPLC.
A packed chromatographic column comprising silica particles inside a column, the packed chromatographic column prepared by the steps of
- a) introducing the silica particles into the column;
- b) ultrasonicating the column;
- and
- c) applying pressure to the silica particles,
- wherein steps a)-c) are performed simultaneously,
- wherein the packed chromatographic column has an eddy diffusion term of less than 100nm;
- wherein the silica particles are uniformly packed and exhibit no cracks or uninterrupted contact between the inner surface of the column and the silica particles, and
- wherein the packed chromatographic column is suitable for HPLC, UHPLC or FPLC.
Dependencies are inferred from the claim text.