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US 9,499,797 B2Granted
Method of making induced pluripotent stem cells
AssigneeKyoto University
InventorsShinya Yamanaka, Keisuke Okita
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Abstract
A method of producing an induced pluripotent stem cell includes introducing into a somatic cell one or more non-viral expression vectors. The vectors include one or more of an Oct family gene, a Klf family gene, a Sox family gene, a Myc family gene, a Lin family gene, and Nanog gene. The somatic cell is then cultured in a medium that supports pluripotent stem cells. At least a portion of the one or more introduced non-viral expression vectors is not substantially integrated in the chromosome.
Claims9
A method of producing an induced pluripotent stem cell, comprising the steps of:
- concurrently introducing two or more plasmids into a mammalian somatic cell in vitro, wherein the two or more plasmids encode the following reprogramming factors:
- Oct 3/4, Sox 2, Klf4, and a Myc family member selected from the group consisting of c-Myc, N-Myc, L-Myc and T58A mutant of c-Myc,
- wherein at least one plasmid vector encodes two or more of the reprogramming factors via an intervening sequence enabling polycistronic expression, and
- culturing the mammalian somatic cell in a medium that supports pluripotent stem cells such that iPS cells are obtained, wherein at least a portion of the one or more plasmids are not integrated into a chromosome of the somatic cell.
1 more element
Dependencies are inferred from the claim text.