PDX1-expressing dorsal and ventral foregut endoderm
AssigneeViaCyte, Inc.
InventorsKevin Allen D'Amour, Alan D. Agulnick, Susan Eliazer, Emmanuel E. Baetge
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Abstract
Disclosed herein are cell cultures comprising dorsal and/or ventral PDX1-positive foregut endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified dorsal and/or ventral PDX1-positive foregut endoderm cells as well as methods for enriching, isolating and purifying dorsal and/or ventral PDX1-positive foregut endoderm cells from other cell types. Methods of identifying differentiation factors capable of promoting the differentiation of dorsal and/or ventral PDX1-positive foregut endoderm cells, are also disclosed.
Claims11
An in vitro cell culture comprising human definitive endoderm cells, human pancreatic-duodenal homeobox factor-1 (PDX1) positive foregut endoderm cells, and a medium comprising an effective amount of a TGFβ superfamily member and an effective amount of a retinoid, wherein the effective amount of a TGFβ superfamily member and retinoid promote differentiation of definitive endoderm cells to PDX1-positive foregut endoderm cells, wherein the TGFβ superfamily member is activin A and wherein the retinoid is retinoic acid.
Dependencies are inferred from the claim text.