Highly sensitive system and method for analysis of troponin
AssigneesSingulex, Inc., The Regents of the University of California
InventorsPhilippe Goix, Robert Puskas, John Todd, Richard Livingston, Douglas Held, Allan H. B. Wu
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Abstract
The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin, Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.
Claims9
A method for determining the presence or absence of cardiac troponin selected from cardiac I (cTnI) or cardiac troponin T (cTnT) or complex thereof at a concentration of less than 3 pg/mL in a biological sample from a healthy human patient, comprising
- i) labeling said troponin or complex thereof, if present, with a label comprising a binding partner for the troponin and a detectable moiety;
- and
- ii) detecting troponin in the sample at concentrations of less than 3 pg/mL by determining the presence or absence of said label in a single molecule counting assay, wherein detection of the presence of said label indicates the presence of said troponin or complex in said sample, wherein said single molecule counting assay has a limit of detection of less than about 3 pg/mL with a coefficient of variation of less than about 10%.
Dependencies are inferred from the claim text.