Hibiscus cannabinus feruloyl-coa:monolignol transferase
AssigneesBoard of Trustees of Michigan State University, The University of British Columbia, Wisconsin Alumni Research Foundation
InventorsCurtis Wilkerson, John Ralph, Saunia Withers, Shawn D. Mansfield
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Abstract
The invention relates to isolated nucleic acids encoding a feruloyl-CoA:monolignol transferase and feruloyl-CoA:monolignol transferase enzymes. The isolated nucleic acids and/or the enzymes enable incorporation of monolignol ferulates into the lignin of plants, where such monolignol ferulates include, for example, p-coumaryl ferulate, coniferyl ferulate, and/or sinapyl ferulate. The invention also includes methods and plants that include nucleic acids encoding a feruloyl-CoA:monolignol transferase enzyme and/or feruloyl-CoA:monolignol transferase enzymes.
Claims23
An isolated Hibiscus cannabinus (Kenaf) feruloyl-CoA:monolignol transferase cDNA with at least 95% sequence identity to nucleic acid sequence SEQ ID NO:8.
An isolated cDNA that encodes a Hibiscus cannabinus (Kenaf) feruloyl-CoA:monolignol transferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:9 or SEQ ID NO:16.
An expression cassette comprising a feruloyl-CoA:monolignol transferase nucleic acid segment with at least about 95% sequence identity to nucleic acid sequence SEQ ID NO:8, and a heterologous promoter operably linked to the nucleic acid segment, wherein the promoter is functional in a host cell.
An expression cassette comprising a nucleic acid segment that encodes a Hibiscus cannabinus (Kenaf) feruloyl-CoA:monolignol transferase polypeptide with at least 95% amino acid sequence identity to SEQ ID NO:9 or SEQ ID NO:16, and a heterologous promoter operably linked to the nucleic acid segment.
A plant comprising a heterologous feruloyl-CoA:monolignol transferase nucleic acid segment with at least about 95% sequence identity to nucleic acid sequence SEQ ID NO:8, and a heterologous promoter operably linked to the nucleic acid segment, wherein the promoter is functional in a cell of the plant.
A plant seed comprising a heterologous feruloyl-CoA:monolignol transferase nucleic acid segment with at least about 95% sequence identity to nucleic acid sequence SEQ ID NO:8, and a promoter operably linked to the nucleic acid segment, wherein the promoter is functional in a cell of the plant.
Dependencies are inferred from the claim text.