Nonribosomal peptide synthetases
AssigneeThe Regents of the University of Michigan
InventorsDavid H. Sherman, Michael Marie Kaufman-Schofield, Sunit Jain, Gregory Dick
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Abstract
The present disclosure is directed to the biosynthetic pathway for a nonribosomal peptide synthetase (NRPS)-derived drug and analogs thereof. The invention provides polynucleotide sequences useful for heterologous expression in a convenient microbial host for the synthesis of the NRPS-derived drug, the polypeptides encoded by such polynucleotides, expression vectors comprising the polynucleotides, host cells comprising the polynucleotides or expression vectors, and kits comprising a host cell. Also provided is a method for the production of ET-743, the NRPS-derived drug.
Claims15
A method for producing ET-743 or a metabolic intermediate thereof comprising:
- growing a host cell transformed with one or more expression vectors comprising a polynucleotide encoding one or more polypeptides selected from the group consisting of SEQ ID NOs:
- 421, 288, 289, 290, 291, 420, 350, 423, 425, 427, 429, 431, 433 and 435 under conditions to express the one or more polypeptides and producing ET-743 or the metabolic intermediate for producing ET-743.
A method for producing ET-743 or a metabolic intermediate thereof comprising:
- expressing at least one heterologous polypeptide encoded by an expression vector in a host cell, in wherein the polypeptide comprises the sequence set forth in any one of SEQ ID NOs:
- 421, 288, 289, 290, 291, 420, 350, 423, 425, 427, 429, 431, 433 or 435 that provides an ET-743 biosynthetic activity, thereby producing ET-743 or the metabolic intermediate thereof.
Dependencies are inferred from the claim text.