Methods and devices for multiplexed microarray microfluidic analysis of biomolecules

A method for microarray-to-microarray transfer, the method comprising:

• inserting an assay chip into a first recess of a first element of a snap apparatus, the assay chip comprising a plurality of first locations disposed on a surface of the assay chip to form a first array, each first location defined by first coordinates relative to a predetermined point on the assay chip, the first recess sized for receiving, aligning, and retaining the assay chip therein, at least one of the first locations comprising a first material;
• inserting a transfer chip into a second recess of a second element of the snap apparatus, the transfer chip comprising a plurality of second locations disposed on a surface of the transfer chip to form a second array, each second location defined by second coordinates relative to a predetermined point on the transfer chip having a predetermined relationship with the predetermined point on the assay chip, the first array and the second array being mirror patterns of each other, the second recess sized for receiving, aligning and retaining the transfer chip therein, at least one of the second locations corresponding to the at least one of the first locations comprising a second material;
• providing a spacer between the assay chip and the transfer chip, the spacer sized to separate the assay chip and the transfer chip by a predetermined distance when the first element and the second element are brought together;
• and
• closing the snap apparatus by bringing the first element and the second element together to form a liquid bridge between the first material and the second material.