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US 9,476,070 B2Granted
Recombinant microorganism with ability to produce glycerol, 3-HP, or acrylic acid and method of producing glycerol, 3-HP, or acrylic acid by using the same
AssigneeSamsung Electronics Co., Ltd.
InventorsWonseok Jung, Eunyoung Kim, Jinho Kang, Nagjong Kim
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Abstract
A recombinant microorganism having the ability to produce glycerol 3-HP, or acrylic acid, in which glycerol is produced from dihydroxyacetone phosphate (DHAP) via dihydroxyacetone (DHA) in a biosynthetic pathway, and a method of producing glycerol, 3-hydroxypropioninc acid (3-HP), or acrylic acid by using the recombinant microorganism.
Claims18
A recombinant microorganism comprising:
- a polynucleotide encoding a haloacid dehydrogenase (HAD) superfamily dihydroxyacetone phosphate phosphatase (DHAPP), that catalyzes the conversion of dihydroxyacetone phosphate (DHAP) into dihydroxyacetone (DHA) and is from Corynebacterium genus and having phosphatase activity P-type ATPase;
- and
- a polynucleotide encoding glycerol dehydrogenase (GLDH) categorized as EC 1.1.1.6 that catalyzes the conversion of DHA into glycerol,
- a polynucleotide encoding glycerol dehydratase (GDH) categorized as EC 4.2.1.30 that catalyzes the conversion of glycerol into 3-hydroxypropionaldehyde (3-HPA);
- and
- a polynucleotide encoding an aldehyde dehydrogenase (ALD) that catalyzes the conversion of 3-HPA into 3-hydroxypropionic acid (3-HP), wherein the ALD is a succinate semialdehyde dehydrogenase (SSADH) categorized as EC 1.2.1.24 or EC 1.2.1.16,
- wherein at least one of the polynucleotides encoding the DHAPP, GLDH, GDH, or ALD are introduced via genetic manipulation, and
- wherein the recombinant microorganism is a recombinant Escherichia microorganism.
5 more elements
A method of making a microorganism that produces glycerol, the method comprising introducing into a microorganism that does not produce glycerol:
- a polynucleotide encoding a haloacid dehydrogenase (HAD) superfamily dihydroxyacetone phosphate phosphatase (DHAPP) that catalyzes the conversion of dihydroxyacetone phosphate (DHAP) into dihydroxyacetone (DHA) and is from Corynebacterium genus and having phosphatase activity of P-type ATPase;
- and
- a polynucleotide encoding glycerol dehydrogenase (GLDH) categorized as EC 1.1.1.6 that catalyzes the conversion of DHA into glycerol;
- a polynucleotide encoding glycerol dehydratase (GDH) categorized as EC 4.2.1.30 that catalyzes the conversion of glycerol into 3-hydroxypropionaldehyde (3-HPA);
- and
- a polynucleotide encoding an aldehyde dehydrogenase (ALD) that catalyzes the conversion of 3-HPA into 3-hydroxypropionic acid (3-HP), wherein the ALD is a succinate semialdehyde dehydrogenase (SSADH) categorized as EC 1.2.1.24 or EC 1.2.1.16,
- thereby providing a microorganism that produces glycerol,
- wherein the microorganism belongs to the genus Escherichia.
5 more elements
Dependencies are inferred from the claim text.