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AssigneesPioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and Company
InventorsDaniel J. Altier, Jennifer K. Barry, Carol A. Hendrick, Lu Liu, Phillip A. Patten, Claudia D. Perez-Ortega, Eric J. Schepers, Weiping Xie, Nasser Yalpani, Jianzhou Zhao, Xiaohong Zhong, Genhai Zhu
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Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
A recombinant nucleic acid molecule encoding an Alcaligenes faecalis Insecticidal polypeptide-1A (AfIP-1A polypeptide) operably linked to a heterologous regulatory element, wherein the AfIP-1A polypeptide has at least 85% identity to the amino acid sequence of SEQ ID NO:
A transgenic plant capable of expressing a recombinant polynucleotide encoding an AfIP-1A polypeptide having at least 85% identity to the amino acid sequence of SEQ ID NO:
A recombinant nucleic acid molecule encoding an Alcaligenes faecalis Insecticidal polypeptide-1B (AIIP-1B polypeptide) operably linked to a heterologous regulatory element, wherein the AfIP-1B polypeptide has at least 95% identity to the amino acid sequence of SEQ ID NO:
A transgenic plant capable of expressing a recombinant polynucleotide encoding an AfIP-1B polypeptide having at least 95% identity to the amino acid sequence of SEQ ID NO:
A method for protecting a plant from an insect pest, comprising expressing in the plant or cell thereof, an insecticidally-effective amount of an AfIP-1A polypeptide having at least 85% identity to the amino acid sequence of SEQ ID NO:
A fusion protein comprising a recombinant AfIP-1A polypeptide having at least 85% identity to the amino acid sequence of SEQ ID NO:
A fusion protein comprising a recombinant AfIP-1B polypeptide having at least 95% identity to the amino acid sequence of SEQ ID NO:
A fusion protein comprising a recombinant AfIP-1A polypeptide having at least 85% identity to the amino acid sequence of SEQ ID NO:
Dependencies are inferred from the claim text.