Process for purification of a fatty acid binding protein
AssigneesOuro Fino Agronegocio Ltda., FundaçãO Oswaldo Cruz
InventorsMiriam Tendler, Celso Raul Romero Ramos, Andrew J. G. Simpson
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Abstract
A process for purification of a fatty acid binding proteins such as, e.g., Sm14 of Pichia pastoris or type-3 FABP protein of Fasciola hepatica. The process includes the steps of: (a) performing lysis of cells containing the fatty acid binding protein to obtain a lysate; (b) clarifying the lysate obtained in step (a) to obtain a clarified lysate; (c) loading the clarified lysate in a column containing an anion exchange resin; (d) eluting proteins from the column by pH changes in the column; and (e) separating contaminant proteins from the fatty acid binding protein by gel-filtration.
Claims7
A process for purification of a recombinant Sm14 Schistosoma mansoni protein, said process comprising the steps of:
- (a) performing lysis of cells containing the Sm14 protein to obtain a lysate;
- (b) clarifying the lysate obtained in step (a) to obtain a clarified lysate;
- (c) loading the clarified lysate in a column containing an anion exchange resin;
- (d) eluting proteins from the column by pH changes in the column;
- and
- (e) separating contaminant proteins from the Sm14 protein by gel-filtration, wherein said Sm14 protein is encoded by SEQ ID NO:3 and is expressed in Pichia pastoris.
Dependencies are inferred from the claim text.