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US 9,469,675 B2Granted
Anti-inflammatory proteins and methods of preparation and use thereof
AssigneeManukaMed Limited
InventorsAmanda Bean, Peter Molan, Ray Cursons, Richard Wilkins
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Abstract
The present disclosure relates to anti-inflammatory proteins, their uses, methods of preparation and methods of their detection. In particular, the invention relates to major royal jelly proteins modified by methyglyoxal and fragments thereof from manuka honey.
Claims9
A method of identifying (i) anti-inflammatory capacity and (ii) MGO-modified apalbumin concentration of a honey sample, comprising the steps of:
- a) measuring the fluorescence level of the honey sample,
- b) determining the concentration of MGO-modified apalbumin (MMA) in the honey sample by:
- comparing the fluorescence level of the honey sample to a standard scale that correlates fluorescence level to MMA-concentration, or
- comparing the fluorescence level of the honey sample to fluorescence levels of one or more control samples of honey with known MMA-concentrations, and
- c) determining the phagocytosis inhibition (PI) activity the honey sample by:
- comparing the fluorescence level of the honey sample to a standard scale that correlates fluorescence value to PI-activity, or
- measuring the PI-activity of the honey sample, and comparing the PI activity of the honey sample to PI-activities of one or more control samples of honey with known PI-activities,
- wherein (b) identifies the concentration of the MGO-modified apalbumin in the honey sample and (c) identifies the anti-inflammatory capacity of the honey sample.
5 more elements
Dependencies are inferred from the claim text.