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US 9,463,458 B2Granted
Diagnostic devices and methods of use
AssigneeDignity Health
InventorJonathan E. Valla
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods of diagnosing samples as well as various microfluidic, microcentrifuge and microfilter devices. In one embodiment, the present invention provides a method of diagnosing neurodegenerative diseases using mitochondrial and/or platelet samples. In another embodiment, the present invention provides a microfluidic device that selectively captures and analyzes a desired amount of target biological particle.
Claims34
A kit for the diagnosis of a neurodegenerative disease, comprising:
- (a) a device, comprising:
- an insert configured to be inserted into a cuvette comprising a mounting slot configured to hold a support fin of the insert,
- wherein the insert comprises a top portion transitioning to a bottom portion,
- wherein the top portion comprises a sample chamber for receiving a sample,
- wherein the bottom portion comprises an optical measurement chamber and the support fin extending along two opposite sides outside of the optical measurement chamber,
- wherein the top portion is wider than the bottom portion, and wherein the top portion accommodates the supernatant of the sample and the bottom portion accommodates the pellet of the sample after the sample is centrifuged;
- and
- (b) instructions for using the device to diagnose a neurodegenerative disease.
5 more elements
A device, comprising:
- an insert configured to be inserted into a cuvette comprising a mounting slot configured to hold a support fin of the insert,
- wherein the insert comprises a top portion transitioning to a bottom portion,
- wherein the top portion comprises a sample chamber for receiving a sample,
- wherein the bottom portion comprises an optical measurement chamber and the support fin extending along two opposite sides outside of the optical measurement chamber,
- wherein the top portion is wider than the bottom portion, and wherein the top portion accommodates the supernatant of the sample and the bottom portion accommodates the pellet of the sample after the sample is centrifuged.
2 more elements
A method of diagnosing a neurodegenerative disease in a subject, comprising:
- providing a device, comprising:
- an insert configured to be inserted into a cuvette comprising a mounting slot configured to hold a support fin of the insert,
- wherein the insert comprises a top portion transitioning to a bottom portion,
- wherein the top portion comprises a sample chamber for receiving a sample,
- wherein the bottom portion comprises an optical measurement chamber and the support fin extending along two opposite sides outside of the optical measurement chamber,
- wherein the top portion is wider than the bottom portion, and wherein the top portion accommodates the supernatant of the sample and the bottom portion accommodates the pellet of the sample after the sample is centrifuged;
- obtaining a sample from the subject;
- quantifying the amount or activity of a target moiety in the sample using the device by centrifuging the sample to sediment the target moiety into the optical measurement chamber of the insert;
- and
- comparing the amount or activity of the target moiety in the sample with the amount or activity of the target moiety in a standard, wherein a change in the amount or activity of the target moiety in the sample relative to the standard indicates that the subject has or will develop the neurodegenerative disease.
7 more elements
Dependencies are inferred from the claim text.