Small molecules supporting pluripotent cell growth and methods thereof
AssigneeViaCyte, Inc.
InventorsThomas C Schulz, Allan J Robins
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Abstract
The present invention relates to compositions and methods for maintaining undifferentiated pluripotent stem cell cultures.
Claims23
An in vitro composition comprising single human pluripotent cells whereby said pluripotent cells are plated as single cells at a low cell density in a medium comprising an effective amount of a neurotransmitter or a structural analog, derivative or physiologically acceptable salt thereof sufficient to achieve low density survival and expansion of the pluripotent cells.
An in vitro suspension culture comprising single human pluripotent cells whereby said pluripotent cells are in suspension as single cells and an effective amount of a neurotransmitter or a structural analog, derivative or physiologically acceptable salt thereof.
A method of expanding human pluripotent cells in vitro comprising:
- a. obtaining single human pluripotent cells;
- and
- b. plating said single human pluripotent cells as single cells at low cell density in a medium comprising an effective amount of a neurotransmitter or a structural analog, derivative or physiologically acceptable salt thereof sufficient to achieve low density survival and expansion of the pluripotent cells.
Dependencies are inferred from the claim text.