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US 9,453,248 B2Granted
Coryneform bacterium transformant and process for producing aniline using the same
AssigneesResearch Institute of Innovative Technology for the Earth, Sumitomo Rubber Industries, Ltd.
InventorsHideaki Yukawa, Masayuki Inui
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Abstract
Provided is an aniline-producing transformant constructed by introducing a gene which encodes an enzyme having aminobenzoate decarboxylase activity into a coryneform bacterium as a host. Also provided is a process for producing aniline, which comprises a step of allowing the transformant to react in a reaction mixture containing aminobenzoic acid, an ester thereof, and/or a salt thereof under reducing conditions, and a step of recovering aniline from the reaction mixture.
Claims6
A process for producing aniline, comprising:
- A) contacting an aniline-producing coryneform bacterium with a reaction mixture comprising aminobenzoic acid, an ester thereof, and/or a salt thereof to produce aniline, and
- B) recovering said aniline from said reaction mixture, wherein said aniline-producing coryneform bacterium is transformed with a polynucleotide encoding an enzyme having aminobenzoate decarboxylase activity, wherein said polynucleotide comprises a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence of SEQ ID NO:
- 16, SEQ ID NO:
- 19, SEQ ID NO;
- 22, SEQ ID NO:
- 25, SEQ ID NO:
- 28, SEQ ID NO:
- 31or SEQ ID NO:
- 34;
- and
- (b) a nucleotide sequence that has at least 95% nucleotide sequence identity with the nucleotide sequence of SEQ ID NO:
- 16, 19, 22, 25, 28, or 31, wherein said nucleotide sequence encodes a polypeptide having aminobenzoate decarboxylase activity.
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Dependencies are inferred from the claim text.