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US 9,447,439 B2Granted
Microorganism producing 4-hydroxybutyrate and a method for producing 4-hydroxybutyrate in anaerobic condition using the same
AssigneeSamsung Electronics Co., Ltd.
InventorsYoungmin Lee, Wooyong Lee, Jinwoo Kim, Jaechan Park, Jinhwan Park, Hwayoung Cho
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Abstract
A genetically modified microorganism comprising a polynucleotide encoding α-ketoglutarate synthase or a mutant thereof, and a polynucleotide encoding pyruvate carboxylase or a mutant thereof; wherein the genetically modified microorganism has decreased malate quinone oxidoreductase activity and/or decreased phosphoenolpyruvate carboxykinase activity compared to an unmodified microorganism of the same type, and wherein the genetically modified microorganism produces 4-hydroxybutyrate.
Claims19
A genetically modified microorganism comprising
- a polynucleotide encoding exogenous α-ketoglutarate synthase, and
- a polynucleotide encoding endogenous pyruvate carboxylase or a mutant thereof;
- wherein the genetically modified microorganism has decreased malate quinone oxidoreductase activity, decreased phosphoenolpyruvate carboxykinase activity, or a combination thereof, compared to an unmodified microorganism of the same type,
- wherein the genetically modified microorganism produces 4-hydroxybutyrate, and
- wherein the genetically modified microorganism is a strain selected from the group consisting of lumen bacteria, Corynebacterium genus, Brevibacterium genus, and Escherichia coli.
2 more elements
Dependencies are inferred from the claim text.