PH20 polypeptide variants, formulations and uses thereof

A modified PH20 polypeptide that exhibits increased stability in the presence of a phenolic preservative, comprising an amino acid replacement in an unmodified PH20 polypeptide, wherein:

• the amino acid replacement confers the increased stability;
• the amino acid replacement is replacement with proline at a position corresponding to position 204 in a PH20 polypeptide with reference to amino acid residue positions set forth in SEQ ID NO:3;
• corresponding amino acid positions are identified by alignment of the modified PH20 polypeptide with the polypeptide whose sequence is set forth in SEQ ID NO:3;
• the modified PH20 polypeptide has at least 85% sequence identity to the sequence of amino acids set forth in SEQ ID NO:
• 3 or 7;
• and
• stability is increased compared to the unmodified polypeptide without the amino acid replacement, wherein stability is measured by resistance to denaturation in the presence of a phenolic preservative.